EFFECT OF POLYSACCHARIDE CAPSULE AND METHODS OF PREPARATION ON HUMAN LYMPHOCYTE-PROLIFERATION IN RESPONSE TO CRYPTOCOCCUS-NEOFORMANS
EFFECT OF POLYSACCHARIDE CAPSULE AND METHODS OF PREPARATION ON HUMAN LYMPHOCYTE-PROLIFERATION IN RESPONSE TO CRYPTOCOCCUS-NEOFORMANS
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DOI：
10.1128/iai.61.2.464-469.1993
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发表时间：
1993-02-01
影响因子：
3.1
通讯作者：
SYME, RM
中科院分区：
文献类型：
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作者：
MODY, CH;SYME, RM
新型隐球菌是一种致病性包囊酵母，感染有缺陷的细胞介导的免疫力的患者，包括艾滋病患者。通过加热杀死的整个隐球菌生物体刺激正常人淋巴细胞增殖。然而，C.新形式病毒在毒力方面变化很大，因此在它们引起人类疾病的能力方面变化很大。确定毒力因子，如隐球菌荚膜、血清型和微生物状态对淋巴细胞对隐球菌应答的影响。人外周血单个核细胞用C.体外培养新生儿并测定淋巴细胞增殖。淋巴细胞对C. neoformans是存在的多糖的量。最小包囊菌株（菌株C3D，68和613）刺激后的反应大于重度包囊菌株（菌株6和145）。一个重包囊菌株（菌株6）没有抑制无包囊突变体（菌株67）的反应。然而，通过加入纯化的多糖抑制了对无囊菌株的反应。人淋巴细胞对两种血清型的C.新形变种新人类尽管采用了各种灭活技术，包括加热、多聚甲醛固定、辐照和机械破坏，但负责淋巴细胞刺激的抗原仍得以保留。最后，淋巴细胞对活的和死的生物体的反应是一样的。这些结果表明，荚膜多糖，一个已知的毒力因子，可能会抑制人淋巴细胞对C。感染期间的新生儿。淋巴细胞可以对C.无论生物体的生存能力如何，它们也可以对被破坏的生物体作出反应。我们推测淋巴细胞的体外增殖可能与宿主防御C. neoformans的毒力因子抑制，新人类
Cryptococcus neoformans is a pathogenic encapsulated yeast that infects patients that have defective cell-mediated immunity, including AIDS patients. Whole cryptococcal organisms that are killed by heating stimulate normal human lymphocytes to proliferate. However, strains of C. neoformans vary widely in virulence and therefore in their ability to cause disease in humans. To determine the effect of virulence factors such as the cryptococcal capsule, serotype, and the state of the organisms on the lymphocyte response to C. neoformans, human peripheral blood mononuclear cells were stimulated with C. neoformans in vitro and lymphocyte proliferation was determined. The major determinant of the lymphocyte response to C. neoformans was the amount of polysaccharide present. The response was greater after stimulation by minimally encapsulated strains (strains C3D, 68, and 613) than by heavily encapsulated strains (strains 6 and 145). A heavily encapsulated strain (strain 6) did not suppress the response to an acapsular mutant (strain 67). However, the response to an acapsular strain was suppressed by the addition of purified polysaccharide. Human lymphocytes responded to both serotypes of C. neoformans var. neoformans. The antigen responsible for lymphocyte stimulation was preserved despite various techniques of inactivation, including heat, paraformaldehyde fixation, irradiation, and mechanical disruption. Finally, lymphocytes responded equally to live and killed organisms. These results suggest that capsular polysaccharide, a known virulence factor, may suppress the human lymphocyte response to C. neoformans during an infection. Lymphocytes could respond to C. neoformans regardless of the viability of the organism, and they could also respond to disrupted organisms. We speculate that lymphocyte proliferation in vitro could be related to the protective immune response in host defense to C. neoformans and that it is suppressed by virulence factors of C. neoformans.