A probe for the versatile analysis and characterization of N-linked oligosaccharides
A probe for the versatile analysis and characterization of N-linked oligosaccharides
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DOI：
10.1021/ac991268f
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发表时间：
2000-04-01
影响因子：
7.4
通讯作者：
Camilleri, P
中科院分区：
文献类型：
--
作者：
Charlwood, J;Birrell, H;Camilleri, P
合成了一种新的荧光探针3-（乙酰氨基）-6-氨基吖啶（AA-Ac），并研究了它在皮摩尔水平上分析N-连接聚糖的适用性。发现AA-Ac是一种优良的N-连接聚糖衍生试剂，其荧光强度至少是其前身2-氨基吖啶酮的两倍。它们也适合于酶促测序和通过MALDI-TOF质谱、自由区毛细管电泳和毛细管电泳/电喷雾电离质谱进行分析。
A novel fluorescent probe, 3-(acetylamino)-6-aminoacridine (AA-Ac), has been synthesized and its applicability to the analysis of picomole levels of N-linked glycans investigated, AA-Ac was found to be an excellent derivatization reagent for N-linked glycans, giving at least twice the intensity of fluorescence as its predecessor 2-aminoacridone, AA-Ac-labeled glycans were analyzed by both normal and reversed-phase HPLC. They were also amenable to enzymatic sequencing and analysis by MALDI-TOF mass spectrometry, free zone capillary electrophoresis, and capillary electrophoresis/electrospray ionization mass spectrometry.