Surface Epitaxial Crystallization-Directed Nanotopography for Accelerating Preosteoblast Proliferation and Osteogenic Differentiation
Surface Epitaxial Crystallization-Directed Nanotopography for Accelerating Preosteoblast Proliferation and Osteogenic Differentiation
复制标题
表面外延结晶定向纳米形貌加速前成骨细胞增殖和成骨分化
DOI：
10.1021/acsami.9b14800
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发表时间：
2019
影响因子：
9.5
通讯作者：
Li Zhong-Ming
中科院分区：
文献类型：
--
作者：
Yin Hua-Mo;Liu Wei;Huang Yan-Fei;Ren Yue;Xu Ling;Xu Jia-Zhuang;Zhao Baisong;Li Zhong-Ming
表面纳米拓扑术提供了一种物理刺激来指导细胞的命运，特别是在成骨分化的情况下。然而，纳米粒子的制造通常需要复杂的程序。本文提出了一种可行且通用的方法，通过表面外延结晶在聚己内酯(ε)基片上制备出独特的纳米薄片。表面纳米片的厚度、周期间距和均方根纳米粗糙度可以通过简单地改变生长溶液中的PCL浓度来调节。外延纳米片具有与衬底相同的成分，这是揭示生物物理连接对图案化表面成骨机制的独立影响的先决条件。从前成骨细胞的增殖和成骨分化方面检测了外延纳米片对前成骨细胞的反应。外延纳米片显著促进碱性磷酸酶、I型胶原、骨桥蛋白、骨钙素的表达和矿化。成骨分化的加速归因于激活TAZ/RUNX2信号通路。结果表明，表面外延结晶法是设计和构建骨组织工程纳米拓扑图的一种可行方法。
Surface nanotopography provides a physical stimulus to direct cell fate, especially in the case of osteogenic differentiation. However, fabrication of nanopatterns usually suffers from complex procedures. Herein, a feasible and versatile method was presented to create unique nanosheets on a poly(ε-caprolactone) (PCL) substrate via surface epitaxial crystallization. The thickness, periodic distance, and root-mean-square nanoroughness of surface nanosheets were tunable by simply altering the PCL concentration in the growth solution. Epitaxial nanosheets possessed an identical composition as the substrate, being a prerequisite to revealing the independent effect of biophysical linkage on the osteogenic mechanism of the patterned surface. Preosteoblasts’ response to the epitaxial nanosheets was examined in the aspect of preosteoblast proliferation and osteogenic differentiation. The expression of alkaline phosphatase, collagen type I, osteopontin, and osteocalcin as well as mineralization was significantly promoted by the epitaxial nanosheets. Acceleration of osteogenic differentiation was attributed to activating the TAZ/RUNX2 signaling pathway. The findings demonstrate that surface epitaxial crystallization is a feasible approach to design and construct nanotopography for bone tissue engineering.