The long-term memory trace formed in the Drosophila α/β mushroom body neurons is abolished in long-term memory mutants.
The long-term memory trace formed in the Drosophila α/β mushroom body neurons is abolished in long-term memory mutants.
复制标题
DOI：
10.1523/jneurosci.3190-10.2011
复制
发表时间：
2011-04-13
期刊：
影响因子：
--
通讯作者：
Davis RL
中科院分区：
文献类型：
--
作者：
Akalal DB;Yu D;Davis RL
之前的一次筛查发现，数十个黑腹果蝇突变体具有缺陷的长期记忆(LTM)。使用间隔嗅觉条件作用，我们训练其中26个突变株将气味提示与电击联系起来，然后在24小时后检查这种条件作用的记忆。所有测试的突变体都显示，与在对照果蝇中观察到的健壮的LTM相比，LTM存在缺陷。我们利用在体功能光学成像来测量先前表征的LTM痕迹的大小，其表现为对条件性气味的反应增加了钙内流到α/β蘑菇体神经元的轴突。这一记忆痕迹在所有26个LTM突变体中都存在缺陷。这些观察提高了这一LTM痕迹的意义，因为26个独立突变体都表现出该痕迹中的缺陷，并进一步表明钙痕迹是果蝇LTM的基本机制。
A prior screen identified dozens of Drosophila melanogaster mutants that possess defective long-term memory (LTM). Using spaced olfactory conditioning, we trained 26 of these mutant lines to associate an odor cue with electric shock and then examined the memory of this conditioning 24 hours later. All of the mutants tested revealed a deficit in LTM compared to the robust LTM observed in control flies. We utilized in vivo, functional optical imaging to measure the magnitude of a previously characterized LTM trace, which is manifested as increased calcium influx into the axons of α/β mushroom body neurons in response to the conditioned odor. This memory trace was defective in all 26 of the LTM mutants. These observations elevate the significance of this LTM trace given that 26 independent mutants all exhibit a defect in the trace, and further suggest that the calcium trace is a fundamental mechanism underlying Drosophila LTM.