GENERATION OF DIVERSE HIGH-AFFINITY HUMAN MONOCLONAL-ANTIBODIES BY REPERTOIRE CLONING
GENERATION OF DIVERSE HIGH-AFFINITY HUMAN MONOCLONAL-ANTIBODIES BY REPERTOIRE CLONING
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DOI：
10.1073/pnas.88.6.2432
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发表时间：
1991-03-01
影响因子：
11.1
通讯作者：
BURTON, DR
中科院分区：
文献类型：
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作者：
PERSSON, MAA;CAOTHIEN, RH;BURTON, DR
来自用破伤风类毒素免疫的个体的外周血淋巴细胞的抗体重链和轻链的组合文库已经通过使用噬菌体载体在大肠杆菌中表达。文库的筛选允许鉴定大量对破伤风类毒素特异性的人单克隆Fab片段。最初的研究表明这些抗体中存在相当大的序列多样性。该方法应允许产生许多感兴趣的人单克隆抗体和解剖人体液免疫应答。
Combinatorial libraries of antibody heavy and light chains derived from the peripheral blood lymphocytes of an individual immunized with tetanus toxoid have been expressed in Escherichia coli by using phage lambda-vectors. Screening of the libraries allowed identification of a large number of human monoclonal Fab fragments specific for tetanus toxoid. Initial studies suggested considerable sequence diversity in these antibodies. The method should allow the generation of many human monoclonal antibodies of interest and the dissection of human humoral immune responses.