Pattern of expression of engrailed in relation to gamma-aminobutyric acid immunoreactivity in the central nervous system of the adult grasshopper.
Pattern of expression of engrailed in relation to gamma-aminobutyric acid immunoreactivity in the central nervous system of the adult grasshopper.
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成年蚱蜢中枢神经系统中 engrailed 的表达模式与 γ-氨基丁酸免疫反应性的关系。
DOI：
10.1002/cne.1371
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Jia,XX
中科院分区：
文献类型：
--
作者：
Siegler,MV;Pankhaniya,RR;Jia,XX
通过免疫组织化学方法检测了成年蚱蜢（Schistocerca americana）中胸和后胸神经节神经元中 Engrailed（En）蛋白的表达。每个神经节在背侧不成对中位 (DUM) 组中都有一个位于背侧的 En 阳性神经元簇，其中包括中胸神经节 (T2) 中的一个簇和后胸神经节中的四个簇，每个神经节对应一个簇 (T3、A1、A2、A3)。在腹侧，En 阳性神经元出现在每个神经元的后三分之一处。在 T2 和 T3 中，双侧标记了三个腹侧神经元组。在腹部神经元中，En 阳性的腹侧神经元要少得多。这些也是双侧对称的，但没有以允许与 T2 和 T3 组分配同源性的模式出现。总之，En 阳性神经元约占神经节群的 10%。在双侧组中，与 DUM 组一样，En 表达仅限于中间神经元，这与 En 表达有助于中间神经元表型的某些方面的建议一致。 DUM 组中的 En 阳性神经元也表达 γ-氨基丁酸 (GABA) 免疫反应性。进一步的研究表明，一个En阳性双侧组中的所有神经元和另一双侧组中的一些神经元均呈GABA免疫反应性，但第三个双侧组中的神经元仅呈En阳性。此外，几个离散的神经元簇具有 GABA 免疫反应性，但 En 阴性。提出了一个临时的形态学方案，它将几个神经元簇与其可能的神经母细胞起源联系起来，作为进一步研究神经元谱系组成的基础。 J.Comp。内罗尔。 440：85–96，2001 年。© 2001 Wiley-Liss, Inc.
Engrailed (En) protein expression in neurons of the mesothoracic and metathoracic ganglia of the adult grasshopper, Schistocerca americana, was examined by immunohistochemistry. Each neuromere had a dorsally located cluster of En-positive neurons within the dorsal unpaired median (DUM) group, comprising one cluster in the mesothoracic ganglion (T2) and four clusters in the metathoracic ganglion, one for each component neuromere (T3, A1, A2, A3). Ventrally, En-positive neurons occurred in the posterior one-third of each neuromere. In T2 and T3, three ventral groups of neurons were labeled bilaterally. In the abdominal neuromeres, many fewer ventral neurons were En-positive. These also were bilaterally symmetrical, but did not occur in patterns that allowed assignment of homology with the T2 and T3 groups. Altogether, En-positive neurons comprised roughly 10% of the ganglionic populations. In the bilateral groups, as in the DUM groups, En expression was restricted to interneurons, consistent with the suggestion that En expression contributes to some aspect of interneuronal phenotype. En-positive neurons in the DUM groups also expressed gamma-aminobutyric acid (GABA) immunoreactivity. Further study showed that all neurons in one En-positive bilateral group and some neurons in another bilateral group were GABA immunoreactive, but that neurons in a third bilateral group were En-positive only. Additionally, several discrete clusters of neurons were GABA-immunoreactive but En-negative. A provisional morphological scheme is presented, which relates the several neuronal clusters to their likely neuroblasts of origin, as a basis for further research into the composition of neuronal lineages. J. Comp. Neurol. 440: 85–96, 2001.© 2001 Wiley-Liss, Inc.