Analysis of nucleotide sequence of wheat yellow mosaic virus genomic RNAs
Analysis of nucleotide sequence of wheat yellow mosaic virus genomic RNAs
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DOI：
10.1007/bf02881780
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发表时间：
1999-10
期刊：
影响因子：
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通讯作者：
Jialin Yu;Liying Yan;N. Su;Z. Hou;Dawei Li;Chenggui Han;Lili Yang;Z. Cai;Yi Liu
中科院分区：
文献类型：
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作者：
Jialin Yu;Liying Yan;N. Su;Z. Hou;Dawei Li;Chenggui Han;Lili Yang;Z. Cai;Yi Liu
小麦黄花叶病毒（WYMV）HC分离物用于病毒cDNA合成和测序。结果表明，该病毒RNA1全长7629个核苷酸，编码一个含2407个氨基酸的多聚蛋白，经自溶裂解后，除病毒外壳蛋白外，还可能产生7种蛋白质。RNA 2全长3639个核苷酸，编码903个氨基酸，可能含有两个非结构蛋白。虽然WYMV与小麦梭条花叶病毒（WSSMV）在遗传结构上有相似性，但它们的核苷酸序列和推导的氨基酸序列的同源性分别只有70%和75%。据此，进一步证实了WYMV和WSSMV是两种不同的病毒。
Wheat yellow mosaic virus (WYMV) isolate HC was used for viral cDNA synthesis and sequencing. The results show that the viral RNAl is 7 629 nucleotides encoding a polyprotein with 2 407 amino acids, from which seven putative proteins may be produced by an autolytic cleavage processing besides the viral coat protein. The RNA2 is 3 639 nucleotides and codes for a polyprotein of 903 amino acids, which may contain two putative non-structural proteins. Although WYMV shares a similarity in genetic organization to wheat spindle streak mosaic virus (WSSMV), the identities in their nucleotide sequences or deduced amino acid sequences are as low as 70 % and 75 % respectively. Based on this result, it is confirmed that WYMV and WSSMV are different species withinBymmirus.