Potential contributions of heat shock proteins and related genes in sexual differentiation in yellow catfish (Pelteobagrus fulvidraco)
Potential contributions of heat shock proteins and related genes in sexual differentiation in yellow catfish (Pelteobagrus fulvidraco)
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DOI：
10.1007/s10695-016-0303-6
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发表时间：
2017-02
影响因子：
2.9
通讯作者：
Yan He;Jie Fang;Liyao Xue;Junjie Wu;F. U. Dawar;Jie Mei
中科院分区：
文献类型：
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作者：
Yan He;Jie Fang;Liyao Xue;Junjie Wu;F. U. Dawar;Jie Mei
体外温动物的性别决定和分化是一个非常复杂的问题，通常受到遗传和环境因素的共同影响。热休克蛋白（HSPs）由于其对温度敏感的表达，是温度依赖性性别决定（TSD）的良好候选者。与大多数对温度敏感的鱼类相似，黄颡鱼（Pelteobagrus fulvidraco）的雌雄比例随温度的升高而增加。黄颡鱼也是一种性别大小二型鱼，雄性个体的生长速度比同龄雌性快得多。因此，研究黄颡鱼的性别分化在水产养殖中具有重要意义。在本研究中，我们利用454焦磷酸测序和Solexa测序技术从黄颡鱼转录组中共鉴定出7个热休克蛋白及其相关基因，其中5个基因具有完整的开放阅读框架（ORF）。在系统发生学上，所有这些基因都与其他脊椎动物的对应基因进行了比较。这些基因在性腺中的表达存在性别差异，其中Hspa 5、Hip和Cdc 37在卵巢中的表达高于睾丸，而Hsp 90 α、Hspb 2、Hspb 8和Hspbp 1在睾丸中的表达高于卵巢。此外，分别在17α-甲基睾酮（MT）和17α-炔雌醇（EE 2）处理后评估了这些基因的表达。本研究结果表明，这些热休克蛋白及其相关基因可能作为辅助分子伴侣，调控性类固醇激素受体的活性，从而影响黄颡鱼性腺的发育。本研究为深入了解硬骨鱼类的性分化机制提供了理论依据。
Sex determination and differentiation in ectotherms are very complicated affairs and usually affected by both genetic and environmental factors. Because of their temperature-sensitive expression, heat shock proteins (HSPs) are good candidates for temperature-dependent sex determination (TSD). Similar to most thermosensitive fish species, the male to female ratio increases with temperature in yellow catfish (Pelteobagrus fulvidraco). Yellow catfish is also a type of sexual size dimorphic fish, and the male individuals grow much faster than females of the same age. Therefore, research of sex differentiation in yellow catfish is important in aquiculture. In this attempt, a total of seven HSPs and related genes were identified from transcriptomes of yellow catfish by 454 pyrosequencing and Solexa sequencing that we did previously, including five genes with complete open reading frame (ORF). Phylogenetically, all these genes were compared with their counterparts from other vertebrates. All these genes were sex-biased expressed in gonads.Hspa5,Hip,andCdc37were expressed more highly in ovary than in testis, whereasHsp90α,Hspb2,Hspb8,andHspbp1were expressed more highly in testis than in ovary. Additionally, the expression of these genes was assessed after 17α-methyltestosterone (MT) and 17α-ethinylestradiol (EE2) treatment, respectively. Our result showed that working as co-chaperones, these HSPs and related genes may regulate sex steroid receptor activities to influence gonad development in yellow catfish. Our work would help in the understanding of the mechanism of sexual differentiation in teleosts.