Identification and quantification of thymosin β4 in human saliva and tears
Identification and quantification of thymosin β4 in human saliva and tears
复制标题
DOI：
10.1196/annals.1415.046
复制
发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Goldstein, Allan L.
中科院分区：
文献类型：
--
作者：
Badamchian, Mahnaz;Damavandy, Ali A.;Goldstein, Allan L.
胸腺素β（4）（T β（4））是一种普遍存在的天然存在的43个氨基酸的肽，其参与多种生物活性，包括血管生成、抑制炎症、伤口愈合、趋化性和内皮细胞迁移。最近的研究还表明，T β（4）加速角膜伤口愈合和下调几种促炎趋化因子和细胞因子。在这项研究中，我们试图确定T β（4）是否天然存在于人类眼泪和其他人类体液中，如唾液。通过EIA分析泪液和唾液样本，以确定和定量T β（4）的含量。从三个不同年龄组中的每一个收集大约10-20个样品：15-20岁、25-35岁和> 50岁，其中眼泪和唾液分别为n = 30和n = 60。排除标准包括在过去6个月内使用任何局部眼科或局部口服药物和/或眼部或口腔手术史。使用Schirmer条从双眼收集眼泪。将唾液样品收集在无菌管中，然后离心以除去固体颗粒。在所有年龄组的泪液和唾液样本中都发现了T β 4。泪液中的浓度范围为0.5-7 μ g/mL，唾液中的浓度范围为0.2-3.6 μ g/mL。在两种液体中，T β 4浓度随年龄而变化，在25-35岁时达到峰值。研究正在进行中，以确定唾液和泪液中的T β（4）水平是否在24小时内表现出昼夜节律，以及确认它们是否随年龄而变化，并探索它们是否随疾病状态而变化。这是第一项报告人类泪液和唾液中存在T β 4的研究。这一发现提出了T β 4作为内源性因子促进角膜和口腔伤口快速愈合的可能性。考虑到T β（4）促进上皮再生并调节抗炎介质，T β（4）可潜在地用于治疗（a）眼表疾病和眼损伤和（B）各种口腔疾病，如牙周病。
Thymosin beta(4) (T beta(4)) is a ubiquitous, naturally occurring, 43-amino acid peptide that takes part in several biological activities including angiogenesis, inhibition of inflammation, wound healing, chemotaxis, and endothelial cell migration. Recent studies also indicate that T beta(4) accelerates corneal wound healing and downregulates several proinflamatory chemokines and cytokines. In this study, we sought to determine whether T beta(4) is naturally occurring in human tears and other human bodily fluids, such as saliva. Tear and saliva samples were analyzed by EIA to identify and quantify the amount of T beta(4) present. Around 10-20 samples were collected from each of three different age groups: 15-20, 25-35, and > 50 years old with n = 30 and n = 60 for tears and saliva, respectively. Exclusion criteria included the use of any topical ophthalmic or topical oral medication and/or history of ocular or oral surgery within the past 6 months. Tears were collected from both eyes using Schirmer's strips. Saliva samples were collected in sterile tubes and were then centrifuged to remove solid particles. T beta(4) was found in tear and saliva samples in all age groups. The concentrations ranged from 0.5-7 mu g/mL in tears and 0.2-3.6 mu g/mL in saliva. In both fluids, T beta(4) concentration varied with age and appeared to peak at ages 25-35 years. Studies are in progress to determine if T beta(4) levels in saliva and tears demonstrate a circadian rhythm during a 24-h period, as well as to confirm that they vary with age and to explore if they vary with diseased states. This is the first study to report the presence of T beta(4) in human tears and saliva. This finding raises the possibility that T beta(4) acts as an endogenous agent contributing to the rapid healing of corneal and oral wounds. Considering that T beta(4) facilitates reepithelialization and modulates anti-inflammatory mediators, T beta(4) could potentially be used therapeutically in the treatment of (a) ocular surface disease and injury of eye and (b) various oral disorders, such as periodontal disease.