A single morphogenetic field gives rise to two retina primordia under the influence of the prechordal plate.
A single morphogenetic field gives rise to two retina primordia under the influence of the prechordal plate.
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发表时间：
1997-02
期刊：
影响因子：
4.6
通讯作者：
Hua Shun Li;Christopher Tierney;L. Wen;Jane Y. Wu;Yi Rao
中科院分区：
文献类型：
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作者：
Hua Shun Li;Christopher Tierney;L. Wen;Jane Y. Wu;Yi Rao
在脊椎动物胚胎中，两侧对称的眼睛起源于前神经板。一个有趣的问题是，两只眼睛是否共享一个共同的发育起源，或者它们分别起源。我们在这里报告，一个新的基因ET的表达模式表明，有一个单一的视网膜领域，解决成两个独立的原基，支持的爪蟾Pax-6基因的表达模式的建议。谱系追踪实验表明，视网膜视野分辨率不是由于在中间区域的细胞迁移到视野的侧部。去除前索中胚层导致形成一个单一的视网膜在鸡胚和爪蟾外植体。鸡胚移植实验表明，前索板能够抑制Pax-6的表达。我们的研究结果提供了直接的证据，存在一个单一的视网膜领域，表明视网膜领域解决抑制视网膜形成的领域的中间区域，并表明，前索板起着主要的信号作用，在视网膜领域的分辨率。
Two bilaterally symmetric eyes arise from the anterior neural plate in vertebrate embryos. An interesting question is whether both eyes share a common developmental origin or they originate separately. We report here that the expression pattern of a new gene ET reveals that there is a single retina field which resolves into two separate primordia, a suggestion supported by the expression pattern of the Xenopus Pax-6 gene. Lineage tracing experiments demonstrate that retina field resolution is not due to migration of cells in the median region to the lateral parts of the field. Removal of the prechordal mesoderm led to formation of a single retina both in chick embryos and in Xenopus explants. Transplantation experiments in chick embryos indicate that the prechordal plate is able to suppress Pax-6 expression. Our results provide direct evidence for the existence of a single retina field, indicate that the retina field is resolved by suppression of retina formation in the median region of the field, and demonstrate that the prechordal plate plays a primary signaling role in retina field resolution.