Dopamine neurons mediate a fast excitatory signal via their glutamatergic synapses
Dopamine neurons mediate a fast excitatory signal via their glutamatergic synapses
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DOI：
10.1523/jneurosci.4317-03.2004
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发表时间：
2004-01-28
影响因子：
5.3
通讯作者：
Rayport, S
中科院分区：
文献类型：
--
作者：
Chuhma, N;Zhang, H;Rayport, S
多巴胺神经元被认为传递一种快速的、激励性的显着信号，比多巴胺介导的信号更快。这个矛盾的解决方案可能是中脑多巴胺神经元发挥快速兴奋作用。使用带有荧光多巴胺神经元的转基因小鼠，其中神经元的轴突投射是可见的，我们制作了包含中脑多巴胺投射的水平脑切片。局灶性细胞外刺激腹侧被盖区的多巴胺神经元诱发多巴胺释放和突触后核神经元的早期单突触和晚期多突触兴奋性反应。局部灌流腹侧被盖区与谷氨酸，这应该激活多巴胺神经元选择性，产生了兴奋性突触事件的增加。局部灌流的腹侧被盖区与D2激动剂quinpirole，这应该增加多巴胺神经元激活的阈值，抑制了早期的反应。所以多巴胺神经元与多巴胺神经元之间形成了多巴胺能突触连接。我们认为，多巴胺神经元的突触传递可能是激励性突显信号的初始成分。
Dopamine neurons are thought to convey a fast, incentive salience signal, faster than can be mediated by dopamine. A resolution of this paradox may be that midbrain dopamine neurons exert fast excitatory actions. Using transgenic mice with fluorescent dopamine neurons, in which the axonal projections of the neurons are visible, we made horizontal brain slices encompassing the mesoaccumbens dopamine projection. Focal extracellular stimulation of dopamine neurons in the ventral tegmental area evoked dopamine release and early monosynaptic and late polysynaptic excitatory responses in postsynaptic nucleus accumbens neurons. Local superfusion of the ventral tegmental area with glutamate, which should activate dopamine neurons selectively, produced an increase in excitatory synaptic events. Local superfusion of the ventral tegmental area with the D2 agonist quinpirole, which should increase the threshold for dopamine neuron activation, inhibited the early response. So dopamine neurons make glutamatergic synaptic connections to accumbens neurons. We propose that dopamine neuron glutamatergic transmission may be the initial component of the incentive salience signal.