The growth profiles of three types of canine distemper virus on vero cells expressing canine signaling lymphocyte activation molecule
The growth profiles of three types of canine distemper virus on vero cells expressing canine signaling lymphocyte activation molecule
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DOI：
10.1292/jvms.67.491
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发表时间：
2005-05-01
影响因子：
1.2
通讯作者：
Tateyama, S
中科院分区：
文献类型：
--
作者：
Lan, NT;Yamaguchi, R;Tateyama, S
为了了解犬瘟热病毒 (CDV) 在稳定表达犬信号淋巴细胞激活分子 (Vero-DogSLAMtag；Vero-DST 细胞) 的 Veto 细胞上的生长情况，在 Vero-DST 细胞中测试了三种 CDV 株的繁殖情况，并与亲本 Vero 细胞进行比较。 MD77 菌株在两种细胞系中均可生长良好，但在 Vero 细胞中未表现出合胞体形成或难以区分的圆形细胞病变效应 (CPE)。 Onderstepoort 和 KDK-1 菌株在具有明显合胞体 CPE 的 Vero-DST 细胞中也生长良好，而它们在 Vero 细胞中分别生长效率较低或无效率。所有三种 CDV 毒株在达到广泛 CPE 之前在 Vero-DST 细胞中均表现出峰值滴度，并且在完全 CPE 时/之后滴度急剧下降。免疫组织化学显示，所有 CDV 毒株的病毒抗原均仅在 Vero-DST 细胞的合胞体中发现，而在 Vero 细胞中，在 MD77 毒株的单个细胞中发现了病毒抗原，但在其他毒株中未发现病毒抗原。因此，每种 CDV 菌株都可以在 Vero-DST 细胞中良好生长，并且针对 Vero 细胞表现不同。这些结果对CDV工作者具有实用价值，因为1）在Vero-DST细胞中，通过观察不同的合胞体CPE，可以识别病毒的最高滴度或最佳生长：2）在Vero细胞中，各种CDV毒株在Vero-DST细胞中繁殖后可以很容易地进行分类。
To know growth profiles of canine distemper virus (CDV) on Veto cells stably expressing canine signaling lymphocyte activation molecule (Vero-DogSLAMtag; Vero-DST cells), the propagation of three strains of CDV was tested in Vero-DST cells in comparison with parental Vero cells. Strain MD77 could grow well in both cell lines, but demonstrated no syncytium formation or indistinguishable rounding cytopathic effects (CPE) in Vero cells. Strains Onderstepoort and KDK-1 also grew well in Vero-DST cells with apparent syncytium CPE, while they grew less or no efficiently, respectively, in Vero cells. All three CDV strains demonstrated the peak titers, in Vero-DST cells before reaching to an extensive CPE and drastic decrease of titers at/after full CPE. Immunohistochemistry revealed that viral antigens of all CDV strains were found exclusively in the syncytia in Vero-DST cells, while in Vero cells, viral antigen was identified in their single cells for strain MD77 but none for other strains. Thus, every strain of CDV could grow well in Vero-DST cells and behaved differently against Vero cells. These results would be of practical value for workers of CDV because 1) In Vero-DST cells, by observation of distinct syncytium CPE, the highest titer or the best growth of virus could be identified: 2) In Vero cells, various CDV strains could be readily classified after propagation in Vero-DST cells.