Extraretinal and retinal hedgehog signaling sequentially regulate retinal differentiation in zebrafish.
Extraretinal and retinal hedgehog signaling sequentially regulate retinal differentiation in zebrafish.
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DOI：
10.1016/s0012-1606(03)00121-0
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发表时间：
2003-06
影响因子：
2.7
通讯作者：
D. L. Stenkamp;R. A. Frey
中科院分区：
文献类型：
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作者：
D. L. Stenkamp;R. A. Frey
刺猬（Hh）信号是脊椎动物眼睛发育所需的;已知的作用包括调节眼睛形态发生以及神经节细胞和感光细胞分化。我们采用了时间选择性的Hh信号敲低策略，通过使用反义吗啉代寡核苷酸或致畸生物碱环巴胺，以剖析Hh信号产生的特定来源的单独作用。我们还研究了眼睛表型的斑马鱼慢肌肉省略（smu）突变体，缺乏一个功能smoothened基因，编码的Hh信号转导途径的组成部分。我们发现，Hh信号从视网膜外的来源是必需的视网膜分化的启动，但这种参与可能是独立的Hh信号对视柄发育的影响。我们还发现来自神经节细胞的Hh信号参与ath 5的增殖表达，并且我们认为来自视网膜色素上皮的Hh信号对光感受器分化的影响可能是由转录因子rx 1介导的。
Hedgehog (Hh) signaling is required for eye development in vertebrates; known roles in the zebrafish include regulation of eye morphogenesis and ganglion cell and photoreceptor differentiation. We employed a temporally selective Hh signaling knockdown strategy, by using antisense morpholino oligonucleotides or the teratogenic alkaloid cyclopamine, in order to dissect the separate roles of Hh signaling arising from specific sources. We also examined the eye phenotype of zebrafish slow muscle-omitted (smu) mutants, which lack a functional smoothened gene, encoding a component of the Hh signal transduction pathway. We find that Hh signaling from extraretinal sources is required for the initiation of retinal differentiation, but this involvement may be independent of the effects of Hh signaling on optic stalk development. We also find that Hh signals from ganglion cells participate in propagating expression of ath5, and we suggest that the effects of Hh signals from the retinal pigmented epithelium on photoreceptor differentiation may be mediated by the transcription factor rx1.