Identification and classification of differentially expressed genes in renal cell carcinoma by expression profiling on a global human 31,500-element cDNA array
Identification and classification of differentially expressed genes in renal cell carcinoma by expression profiling on a global human 31,500-element cDNA array
复制标题
DOI：
10.1101/gr.184501
复制
发表时间：
2001-11-01
期刊：
影响因子：
7
通讯作者：
Poustka, A
中科院分区：
文献类型：
--
作者：
Boer, JM;Huber, WK;Poustka, A
我们利用31,500个元件的互补DNA阵列研究了伴随肾癌发生发展的基因表达的变化。我们测量了37个个体的成对肿瘤和非肿瘤肾上皮样本的表达谱。使用为排除技术和生物噪声而优化的实验设计，以及经过调整的统计测试，我们发现了1738个差异表达的cDNA，预期有6个假阳性。这些基因的功能注释提供了肾癌特定生物通路活动变化的观点。细胞黏附、信号转导和核苷酸代谢是肾癌的生物学过程，其中有很大比例的基因过度表达。肾肿瘤细胞中下调的途径包括小分子转运、离子稳态以及氧和自由基代谢。我们的表达谱数据揭示了与其他上皮性肿瘤相同的基因表达变化，以及肾细胞癌的独特特征。
We investigated the changes in gene expression accompanying the development and progression of kidney cancer by use of 31,500-element complementary DNA arrays. We measured expression profiles for paired neoplastic and noncancerous renal epithelium samples from 37 individuals. Using an experimental design optimized for factoring out technological and biological noise, and an adapted statistical test, we found 1738 differentially expressed cDNAs with an expected number of six false positives. Functional annotation of these genes provided views of the changes in the activities of specific biological pathways in renal cancer. Cell adhesion, signal transduction, and nucleotide metabolism were among the biological processes with a large proportion of genes overexpressed in renal cell carcinoma. Down-regulated pathways in the kidney tumor cells included small molecule transport, ion homeostasis, and oxygen and radical metabolism. Our expression profiling data uncovered gene expression changes shared with other epithelial tumors, as well as a unique signature for renal cell carcinoma.