Efficient transformation of rat 3Y1 cells by human adenovirus type 9.
Efficient transformation of rat 3Y1 cells by human adenovirus type 9.
复制标题
人9型腺病毒有效转化大鼠3Y1细胞。
DOI：
10.1016/0042-6822(84)90169-7
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发表时间：
1984
期刊：
影响因子：
3.7
通讯作者：
Chinnadurai,G
中科院分区：
文献类型：
--
作者：
Brusca,JS;Jannun,R;Chinnadurai,G
人腺病毒9型（D组）在雌性大鼠中以高频率诱导乳腺纤维腺瘤。Ad 9已用于转化已建立的大鼠胚胎成纤维细胞系3 Y1。这些细胞被Ad 9非常有效地转化，转化频率比2型腺病毒（C组）高约16倍。分析了三种转化细胞系（92.2、93.1和96.2）的病毒DNA序列含量和表达。Ad 9转化的细胞系含有约7至30个整合的病毒DNA拷贝。发现所有或几乎所有的病毒基因组以线性形式整合，并且可能以头-尾方向的串联重复序列存在。在这方面，Ad 9转化的细胞类似于Ad 12转化的细胞，其也含有整合的几乎完整的病毒基因组拷贝。已显示Ad 9转化的细胞在RNA水平表达E1 A和E1 B区域。
Human adenovirus type 9 (group D) induces mammary fibroadenomas in female rats at a high frequency. Ad9 has been used to transform an established rat embryo fibroblast cell line, 3Y1. These cells were transformed very efficiently by Ad9 and the frequency of transformation was about 16 times higher than that of adenovirus type 2 (group C). Three transformed cell lines (92.2, 93.1, and 96.2) were analyzed for the content and expression of viral DNA sequences. The Ad9-transformed cell lines contained about 7 to 30 copies of integrated viral DNA. It was found that all or almost all of the viral genome was integrated in a linear form and probably exists as tandem repeats in a head-to-tail orientation. In this respect, Ad9-transformed cells resemble Ad12-transformed cells which also contain integrated nearly complete copies of the viral genome. The Ad9-transformed cells have been shown to express the E1A and E1B regions at the RNA level.