One-Pot Assembly of Drug-Eluting Silk Coatings with Applications for Nerve Regeneration
One-Pot Assembly of Drug-Eluting Silk Coatings with Applications for Nerve Regeneration
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DOI：
10.1021/acsbiomaterials.3c01042
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发表时间：
2023-12-18
影响因子：
5.8
通讯作者：
Zha，R. Helen
中科院分区：
文献类型：
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作者：
Fink，Tanner D.;Funnell，Jessica L.;Zha，R. Helen
用于组织工程的聚合物支架的临床使用通常遭受它们不能促进强细胞相互作用。用生物分子官能化可以改善结果;然而，目前使用共价化学或物理吸附的官能化方法可能导致生物分子生物活性的损失。在这里，我们展示了一种新的自下而上的方法，用于增强聚（L-乳酸）静电纺丝支架的生物活性，通过与丝素蛋白到纳米涂层的蛋白质有效载荷的界面共组装。在我们的方法中，首先将蛋白质有效载荷加入到含有家蚕衍生的丝素蛋白的水溶液中。然后加入磷酸根阴离子以触发有效载荷和丝纤蛋白的共组装，以及在聚（1-乳酸）纤维表面非共价形成有效载荷-丝纤蛋白涂层。重要的是，共组装过程导致蛋白质有效载荷的均匀分布，加载量取决于溶液中的有效载荷浓度和涂覆时间。这种共组装过程产生比物理吸附方法更大的负载能力，并且有效载荷可以在生理相关条件下随时间释放。我们还表明，涂层共组装过程中可以将神经生长因子和共组装的涂层导致显着更多的神经突延伸比加载通过吸附在大鼠背根神经节外植体培养模型。
Clinical use of polymeric scaffolds for tissue engineering often suffers from their inability to promote strong cellular interactions. Functionalization with biomolecules may improve outcomes; however, current functionalization approaches using covalent chemistry or physical adsorption can lead to loss of biomolecule bioactivity. Here, we demonstrate a novel bottom-up approach for enhancing the bioactivity of poly(l-lactic acid) electrospun scaffolds though interfacial coassembly of protein payloads with silk fibroin into nanothin coatings. In our approach, protein payloads are first added into an aqueous solution withBombyx mori-derived silk fibroin. Phosphate anions are then added to trigger coassembly of the payload and silk fibroin, as well as noncovalent formation of a payload-silk fibroin coating at poly(l-lactic) acid fiber surfaces. Importantly, the coassembly process results in homogeneous distribution of protein payloads, with the loading quantity depending on payload concentration in solution and coating time. This coassembly process yields greater loading capacity than physical adsorption methods, and the payloads can be released over time in physiologically relevant conditions. We also demonstrate that the coating coassembly process can incorporate nerve growth factor and that coassembled coatings lead to significantly more neurite extension than loading via adsorption in a rat dorsal root ganglia explant culture model.