Transcription factor Foxp1 stimulates angiogenesis in adult rats after myocardial infarction.
Transcription factor Foxp1 stimulates angiogenesis in adult rats after myocardial infarction.
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DOI：
10.1038/s41420-022-01180-5
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发表时间：
2022-09-10
影响因子：
7
通讯作者：
She, Qiang
中科院分区：
文献类型：
--
作者：
Wang, Dinghui;Liu, Bin;Xiong, Tianhua;Yu, Wenlong;Yang, Huiping;Wang, Jing;Jing, Xiaodong;She, Qiang
叉头盒蛋白P1 （FoxP1）对心脏发育和新生血管的调节至关重要，但其在心脏血管生成中的潜力尚未被探索。本研究旨在探讨FoxP1在大鼠心肌梗死（MI）模型中的血管生成作用。成年雄性大鼠进行心肌梗死，用慢病毒Foxp1 siRNA敲低Foxp1。内皮细胞增殖、血管生成和心功能也进行了评估。采用细胞划痕法和小管形成法检测人脐静脉内皮细胞（HUVECs）的迁移能力和成管能力。结果显示，与假手术组相比，心肌梗死组FoxP1的表达明显升高。Foxp1敲低可降低Foxp1的表达，减少血管生成，增加胶原沉积。当Foxp1 siRNA慢病毒在HUVECs中敲除Foxp1时，细胞增殖、迁移和管形成能力显著下降。我们的研究表明，FoxP1通过促进内皮细胞的增殖，对心肌梗死后心脏血管生成具有多种有益作用。FoxP1应该被认为是治疗性心脏血管生成的候选基因。
Forkhead box protein P1 (FoxP1) is essential for cardiac development and the regulation of neovascularization, but its potential for cardiac angiogenesis has not been explored. This study aims to investigate the angiogenic role of FoxP1 in a rat model of myocardial infarction (MI). Adult male rats were subjected to MI, and Foxp1 was knocked down with lentivirus FoxP1 siRNA. Endothelial cell proliferation, angiogenesis, and cardiac function were also assessed. Cell scratch assay and tubule formation analysis were used to detect the migration ability and tube formation ability of human umbilical vein endothelial cells (HUVECs). Compared with that in the sham group, results showed that the expression of FoxP1 was significantly increased in the MI group. Foxp1 knockdown decreases FoxP1 expression, reduces angiogenesis, and increases collagen deposition. When Foxp1 was knocked down in HUVECs using FoxP1 siRNA lentivirus, cell proliferation, migration, and tube formation abilities decreased significantly. Our study showed that FoxP1 elicits pleiotropic beneficial actions on angiogenesis in the post-MI heart by promoting the proliferation of endothelial cells. FoxP1 should be considered a candidate for therapeutic cardiac angiogenesis.
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影响因子：
20.1
作者：
Meloni M;Caporali A;Graiani G;Lagrasta C;Katare R;Van Linthout S;Spillmann F;Campesi I;Madeddu P;Quaini F;Emanueli C
通讯作者：
Emanueli C
影响因子：
10.5
作者：
Zhang, Yuzhen;Li, Shanru;Morrisey, Edward E.
通讯作者：
Morrisey, Edward E.
影响因子：
64.8
作者：
SHWEIKI, D;ITIN, A;KESHET, E
通讯作者：
KESHET, E
FOXP1通过抑制内皮细胞中的抑制性引导蛋白含量5B来刺激血管生成。
影响因子：
3.7
作者：
Grundmann S;Lindmayer C;Hans FP;Hoefer I;Helbing T;Pasterkamp G;Bode C;de Kleijn D;Moser M
通讯作者：
Moser M
影响因子：
4.8
作者：
Wang, B;Lin, DJ;Tucker, P
通讯作者：
Tucker, P