Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae
Host Species as a Strong Determinant of the Intestinal Microbiota of Fish Larvae
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宿主物种是鱼类幼虫肠道微生物群的重要决定因素
DOI：
10.1007/s12275-012-1340-1
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发表时间：
2012-02-01
影响因子：
3
通讯作者：
Gong, Yingchun
中科院分区：
文献类型：
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作者：
Li, Xuemei;Yu, Yuhe;Gong, Yingchun
本研究采用变性梯度凝胶电泳（DGGE）技术扩增16 S和18 S rRNA基因，比较了银、草鱼、鳙鱼和团头鲂4种淡水鱼幼鱼肠道细菌群落结构，探讨了宿主种类对肠道菌群的影响。相似性聚类分析表明，根据16 S rRNA基因的相似性，这4种鱼类肠道菌群可分为4个类群，而真核生物18 S rRNA基因没有明显的类群。来自共享环境的水样品含有如由16 S和18 S rRNA基因片段两者指示的独立组的微生物群。使用线性回归模型拟合的秩-丰度图可视化细菌群落结构。结果表明，肠道细菌均匀度在种间差异显著（P
We investigated the influence of host species on intestinal microbiota by comparing the gut bacterial community structure of four cohabitating freshwater fish larvae, silver carp, grass carp, bighead carp, and blunt snout bream, using denaturing gradient gel electrophoresis (DGGE) of the amplified 16S and 18S rRNA genes. Similarity clustering indicated that the intestinal microbiota derived from these four fish species could be divided into four groups based on 16S rRNA gene similarity, whereas the eukaryotic 18S rRNA genes showed no distinct groups. The water sample from the shared environment contained microbiota of an independent group as indicated by both 16S and 18S rRNA genes segments. The bacterial community structures were visualized using rank-abundance plots fitted with linear regression models. Results showed that the intestinal bacterial evenness was significantly different between species (P