Study of the antibacterial properties of antimicrobial peptide MOp2 from Moringa oleifera seeds against S. aureus through transcriptomic techniques
Study of the antibacterial properties of antimicrobial peptide MOp2 from Moringa oleifera seeds against S. aureus through transcriptomic techniques
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DOI：
10.1016/j.lwt.2023.115636
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发表时间：
2023-12-12
影响因子：
6
通讯作者：
Wang，Xuefeng
中科院分区：
文献类型：
--
作者：
Huang，Zhiyuan;Dong，Wenming;Wang，Xuefeng
以往的研究从蛋白质组学水平分析了辣木种子多肽MOp 2对金黄色葡萄球菌的潜在抗菌作用靶点，但其抗菌机制尚未完全阐明。因此，本研究旨在研究MOp 2对S.金黄色。MOp 2处理后，金黄色葡萄球菌中共有579个基因表达上调，564个基因表达下调。基因本体论和京都基因百科全书和基因组分析表明，这些差异表达的基因与细胞膜通透性、pH稳态、能量代谢、细胞壁合成和蛋白质折叠有关。酶活性测定证实了乳酸脱氢酶（LDH），琥珀酸脱氢酶（SDH）和ATP酶的下调，这显着破坏pH稳态和能量代谢。RT-qPCR验证RNA-Seq数据显示，sdh CanddnaK表达下调，opuC、adhE、arc和murA表达上调。其中，dnaK是调控靶蛋白DnaK表达抑制S.金黄色。这些发现在转录组水平上补充了MOp 2的抗菌机制，为促进抗菌肽作为新型防腐剂的应用提供了理论依据。
In the previous study, the potential antibacterial targets of peptide MOp2 fromMoringa oleiferaseeds againstStaphylococcus aureuswere analyzed at the proteomic level, but its antibacterial mechanisms were not fully elucidated. Therefore, the present study aimed to investigate the antibacterial properties of MOp2 againstS. aureus. A total of 579 genes were up-regulated, and 564 genes were down-regulated inS. aureusafter treatment with MOp2. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed that these differentially expressed genes were related to cell membrane permeability, pH homeostasis, energy metabolism, cell wall synthesis, and protein folding. The enzymatic activity assays confirmed the down-regulation of lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), and ATPase, which significantly disrupted the pH homeostasis and energy metabolism. Furthermore, the validation of RNA-Seq data by RT-qPCR revealed that the expression ofsdhCanddnaKwas down-regulated, whereas the expression ofopuC,adhE,arc,andmurAwas up-regulated. Among these,dnaKwas the key gene regulating the expression of the protein target DnaK to inhibit the growth ofS. aureus. These findings complemented the antibacterial mechanism of MOp2 at the transcriptomic level, providing a theoretical basis for promoting the application of AMPs as novel preservatives.