Expression of surfactant protein D in the human gastric mucosa and during Helicobacter pylori infection
Expression of surfactant protein D in the human gastric mucosa and during Helicobacter pylori infection
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DOI：
10.1128/iai.70.3.1481-1487.2002
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发表时间：
2002-03-01
影响因子：
3.1
通讯作者：
Thursz, MR
中科院分区：
文献类型：
--
作者：
Murray, E;Khamri, W;Thursz, MR
幽门螺杆菌导致胃粘膜持续感染，临床结局多种多样。先天免疫分子表面活性蛋白D（SP-D）选择性地结合微生物，诱导聚集和吞噬作用。本研究采用逆转录-PCR和免疫组化方法检测SP-D在胃粘膜中的表达。SP-D存在于腔表面和胃小凹内，在表面表达最大。表达水平在H.幽门相关性胃炎与正常粘膜相比。免疫荧光显微镜观察H. pylori的SP-D凝集素特异性。这些活性导致H. pylori，根据使用霍布森BacTracker测量的曲线速度判断。从三种H.幽门螺杆菌菌株显示以浓度依赖性方式结合SP-D，并且菌株之间的结合亲合力存在显著变化。因此，SP-D可能在对H.幽门感染
Helicobacter pylori establishes persistent infection of gastric mucosa with diverse clinical outcomes. The innate immune molecule surfactant protein D (SP-D) binds selectively to microorganisms, inducing aggregation and phagocytosis. In this study, we demonstrated the expression of SP-D in gastric mucosa by reverse transcription-PCR and immuohistochemical analysis. SP-D is present at the luminal surface and within the gastric pits, with maximal expression at the surface. Levels of expression are significantly increased in H. pylori-associated gastritis compared to those in the normal mucosa. Immunofluorescence microscopy was used to demonstrate binding and agglutination of H. pylori by SP-D in a lectin-specific manner. These activities resulted in a 50% reduction in the motility of H. pylori, as judged on the basis of curvilinear velocity measured by using a Hobson BacTracker. Lipopolysaccharides extracted from three H. pylori strains were shown to bind SP-D in a concentration-dependent manner, and there was marked variation in the avidity of binding among the strains. SP-D may therefore play a significant role in the innate immune response to H. pylori infection.