Lectin-bearing polymerized liposomes as potential oral vaccine carriers
Lectin-bearing polymerized liposomes as potential oral vaccine carriers
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DOI：
10.1023/a:1016030202104
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发表时间：
1996-09-01
影响因子：
3.7
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Chen, HM;Torchilin, V;Langer, R
目的.方法：采用疏水性锚N-戊二酰磷脂酰乙醇胺（NGPE）修饰两种凝集素--欧洲荆豆凝集素I（UEA I）和麦胚凝集素（WGA），并将其与聚合脂质体进行比较。将修饰的凝集素掺入脂质体双层中，随后通过聚合使脂质体稳定。首先用X射线光电子能谱法证实了脂质体表面上凝集素的存在。表面固定的凝集素，然后显示保留其碳水化合物结合活性以及特异性的基础上，在体外聚集测定。最后，测定了凝集素脂质体在小鼠体内的释放效率。约10.5%的UEA I脂质体和5.8%的WGA脂质体从胃肠道被吸收。这些数字显著高于在无凝集素脂质体的情况下观察到的3.2%。同时，UEA I脂质体表现出最有效的派伊尔集合淋巴结靶向三者之间，这直接相关的最高交付efficiencyobserved.Conclusions，这建立了凝集素修饰的脂质体可以促进结合派伊尔集合淋巴结，这将提高派伊尔集合淋巴结靶向交付的效率。所有这些都指出了这些凝集素修饰的脂质体作为口服疫苗的新型载体的潜力。
Purpose. The potential of using lectin-modified polymerized liposomes as Peyer's patch targeted oral delivery vehicles was examined.Methods, Two types of lectins, Ulex Europaeus Agglutinin I (UEA I) and Wheat Germ Agglutinin (WGA), were modified with a hydrophobic anchor N-glutaryl-phosphotidylethanolamine (NGPE). The modified lectins were incorporated into liposome bilayers and the liposomes were subsequently stabilized through polymerization. The presence of the lectins on the liposome surfaces was first confirmed with X-ray photoelectron spectroscopy. Surface-immobilized lectins were then shown to retain their carbohydrate binding activities as well as specificities based on an in vitro aggregation assay. Finally, delivery efficiencies of lectin-bearing liposomes were determined in mice.Results. About 10.5% UEA I liposomes and 5.8% WGA liposomes were taken up from the gastrointestinal tract. These numbers are significantly higher than the 3.2% observed in the case of lectin-free liposomes. At the same time, UEA I liposomes exhibited the most effective Peyer's patch targeting among the three, which directly correlated with the highest delivery efficiency observed.Conclusions, This establishes that lectin modification of liposomes can promote binding to Peyer's patches, which will give improved efficiency for Peyer's patch targeted delivery. All these point to the potential for these lectin-modified liposomes as novel vehicles for oral vaccination.