Heparin-like glycosaminoglycans prevent the infection of measles virus in SLAM-negative cell lines
Heparin-like glycosaminoglycans prevent the infection of measles virus in SLAM-negative cell lines
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DOI：
10.1016/j.antiviral.2008.08.006
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发表时间：
2008-12-01
影响因子：
7.6
通讯作者：
Kai, Chieko
中科院分区：
文献类型：
--
作者：
Terao-Muto, Yuri;Yoneda, Misako;Kai, Chieko
麻疹病毒（MV）的广泛组织嗜性表明它涉及广泛表达的分子。我们构建了表达增强型绿色荧光蛋白（EGFP）的重组MV（rMV-EGFP），并证明rMV-EGFP感染了几种不表达人信号淋巴细胞活化分子（SLAM）的细胞类型（HEK-293、HepG2、Hep3B、Huh 7和WRL68细胞），SLAM是麻疹病毒的细胞受体。在使用抗SLAM单克隆抗体的感染性抑制测定中，HEK-293和HepG2细胞的MV感染没有被抑制，表明MV可以在不使用SLAM的情况下感染细胞。可溶性肝素（HP）以剂量依赖性方式抑制SLAM阴性细胞系中的rMV-EGFP感染性。在表面等离子体共振测定中检测纯化的病毒粒子和HP之间的直接相互作用。我们还证明了血凝素（H）蛋白，而不是融合（F）蛋白是负责病毒粒子和HP之间的相互作用。加在一起。我们的结果表明HP样糖胺聚糖与MV的H蛋白结合，并在SLAM阴性细胞的感染中起关键作用。(C)2008 Elsevier B.V.保留所有权利。
The wide tissue tropism of the measles virus (MV) suggests that it involves ubiquitously expressed molecules. We have constructed a recombinant MV expressing the enhanced green fluorescent protein (EGFP) (rMV-EGFP) and demonstrated that the rMV-EGFP infected several cell types (HEK-293, HepG2, Hep3B, Huh7, and WRL68 cells) that do not express the human signalling lymphocyte activation molecule (SLAM), which is known as a cellular receptor for morbilliviruses. MV infection of HEK-293 and HepG2 cells was not inhibited in an infectivity-inhibition assay using an anti-SLAM monoclonal antibody, indicating that MV could infect cells without using SLAM. Soluble heparin (HP) inhibited the rMV-EGFP infectivity in SLAM-negative cell lines in a dose-dependent manner. Direct interaction between purified virions and HP was detected in a surface plasmon resonance assay. We also demonstrated that the hemagglutinin (H) protein, but not the fusion (F) protein is responsible for the interaction between the virions and HP. Taken together. our results suggest that HP-like glycosaminoglycans bind to the H protein of MV and play a key role in the infection of SLAM-negative cells. (C) 2008 Elsevier B.V. All rights reserved.