Enzyme-coated carbon nanotubes as single-molecule biosensors
Enzyme-coated carbon nanotubes as single-molecule biosensors
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DOI：
10.1021/nl034139u
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发表时间：
2003-06-01
期刊：
影响因子：
10.8
通讯作者：
Dekker, C
中科院分区：
文献类型：
--
作者：
Besteman, K;Lee, JO;Dekker, C
我们演示了单个半导体单壁碳纳米管作为多功能生物传感器的使用。氧化还原酶葡萄糖氧化酶(GOx)通过连接分子被控制地附着在纳米管的侧壁上，并被发现引起电导的明显变化。研究发现，酶涂膜管可作为pH传感器，当pH变化时，电导会发生较大且可逆的变化。在添加GOX底物葡萄糖后，可以实时监测阶梯式反应，这表明我们的传感器也能够在单个纳米管的水平上测量酶活性。这种基于纳米管的生物传感器的首次展示为酶研究提供了一种新的工具，并为生物分子诊断开辟了道路。
We demonstrate the use of individual semiconducting single-wall carbon nanotubes as versatile biosensors. Controlled attachment of the redox enzyme glucose oxidase (GOx) to the nanotube sidewall is achieved through a linking molecule and is found to induce a clear change of the conductance. The enzyme-coated tube is found to act as a pH sensor with large and reversible changes in conductance upon changes in pH. Upon addition of glucose, the substrate of GOx, a steplike response can be monitored in real time, indicating that our sensor is also capable of measuring enzymatic activity at the level of a single nanotube. This first demonstration of nanotube-based biosensors provides a new tool for enzymatic studies and opens the way to biomolecular diagnostics.