Discrimination of basal cell carcinoma from normal dermal stroma by quantitative multiphoton imaging
Discrimination of basal cell carcinoma from normal dermal stroma by quantitative multiphoton imaging
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DOI：
10.1364/ol.31.002756
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发表时间：
2006-09-15
期刊：
影响因子：
3.6
通讯作者：
Dong, Chen-Yuan
中科院分区：
文献类型：
--
作者：
Lin, Sung-Jan;Jee, Shiou-Hwa;Dong, Chen-Yuan
我们对人基底细胞癌样本进行了多光子荧光(MF)和二次谐波产生(SHG)成像。在真皮中，基底细胞癌可以通过大量具有较大核和明显的外围栅栏的自发荧光细胞来识别。在正常真皮中，真皮胶原蛋白的倍频信号对多光子信号有很大贡献。然而，在癌症间质中，SHG信号减弱并被自动荧光信号所取代，这表明负责SHG的正常胶原结构已经改变。为了更好地区分癌细胞和癌细胞间质与正常真皮的区别，提出了一种定量的MF/SHG指数。我们证明这一指标可以用来区分癌细胞和邻近的癌间质与正常真皮。我们的工作表明，MF和SHG成像可以作为MOBS手术的替代方案，在安全切除基底细胞癌的实时指导下进行。(C)2006年美国光学学会。
We performed multiphoton fluorescence (MF) and second-harmonic generation (SHG) imaging on human basal cell carcinoma samples. In the dermis, basal cell carcinomas can be identified by masses of autofluorescent cells with relatively large nuclei and marked peripheral palisading. In the normal dermis, SHG from dermal collagen contributes largely to the multiphoton signal. However, within the cancer stroma, SHG signals diminish and Are replaced by autofluorescent signals, indicating that normal collagen structures responsible for SHG have been altered. To better delineate the cancer cells and cancer stroma from the normal dermis, a quantitative MF to SHG index is developed. We demonstrate that this index can be used to differentiate cancer cells and adjacent cancer stroma from the normal dermis. Our work shows that MF and SHG imaging can be an alternative for Mobs' surgery in the real-time guidance of the secure removal of basal cell carcinoma. (c) 2006 Optical Society of America.