Cobalt-containing spherical glass nanoparticles for therapeutic ion release
Cobalt-containing spherical glass nanoparticles for therapeutic ion release
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DOI：
10.1111/jace.17916
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发表时间：
2021-05-27
影响因子：
3.9
通讯作者：
Pereira, Marivalda de M.
中科院分区：
文献类型：
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作者：
Barrioni, Breno R.;de Carvalho, Sandhra M.;Pereira, Marivalda de M.
生物活性玻璃纳米粒(BGNPs)可被细胞内化，使溶出产物在细胞内释放，具有治疗作用。不同的治疗离子可以被加入到玻璃网络中，通过模拟缺氧条件和随后激活促血管生成基因来促进血管生成。采用改进的Stober法制备了单分散的球形致密BGNPs，其组成为SiO_2-CaO-CoO，直径为92+/-1 nm，钴为促血管生成离子。X射线光电子能谱和Si-29固体幻角旋转核磁共振分别证实了Co2+物种的存在和Co、Ca作为网络修饰剂的作用。(4，5-二甲基噻唑-2-基)-2，5-二苯基四氮唑溴化物对直接接触的人骨肉瘤细胞无细胞毒性。这项研究表明，Co2+离子可以被整合到致密的球形BGNPs中，这些材料显示出巨大的潜力，可以作为具有治疗性质的细胞内离子输送系统。
Bioactive glass nanoparticles (BGNPs) can be internalized by cells, allowing the intracellular release of dissolution products with therapeutic benefit. Different therapeutic ions can be incorporated into the glass network that can promote angiogenesis via simulation of hypoxia conditions and consequent activation of pro-angiogenic genes. Here, novel monodispersed spherical dense BGNPs were obtained by a modified Stober method with the SiO2-CaO-CoO composition, with diameters of 92 +/- 1 nm, with cobalt as the pro-angiogenic ion. The presence of Co2+ species and the role of Co and Ca as network modifiers in the silica glass were confirmed by X-ray photoelectron spectroscopy and Si-29 solid-state magic-angle spinning nuclear magnetic resonance, respectively. Controlled Co2+ ion release was observed in culture media, and no cytotoxicity was observed by (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide cell viability assay on human osteosarcoma cells in direct contact with the nanoparticles. This study demonstrated that Co2+ ions can be incorporated into dense and spherical BGNPs, and these materials exhibit great potential as intracellular ion delivery systems with therapeutic properties.