Engineered membrane superchannel improves bioremediation potential of dioxin-degrading bacteria
Engineered membrane superchannel improves bioremediation potential of dioxin-degrading bacteria
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DOI：
10.1038/nbt1181
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发表时间：
2006-02-01
影响因子：
46.9
通讯作者：
Murata, K
中科院分区：
文献类型：
--
作者：
Aso, Y;Miyamoto, Y;Murata, K
鞘氨单胞菌A1具有称为“超通道”的特殊膜结构，可以将大分子直接结合到细胞中。我们设计了两种相关的鞘单胞菌，降解二恶英的S. wittichii RW1和降解聚丙烯乙二醇的S. subarctic IFO 16058(T)，将这种超级通道整合到它们的细胞膜中。在这两种情况下，生物体的生物修复能力都大大提高，这表明这种方法作为一种改善细菌降解环境中有害化合物的一般策略的潜力。
Sphingomonas sp. A1 possesses specialized membrane structures termed 'superchannels' that enable the direct incorporation of macromolecules into the cell. We have engineered two related sphingomonads, the dioxin-degrading S. wittichii RW1 and the polypropylene glycol-degrading S. subarctica IFO 16058(T), to incorporate this superchannel into their cell membranes. In both cases the bioremediation capability of the organisms was substantially increased pointing at the potential of this approach as a general strategy to improve bacterial degradation of hazardous compounds in the environment.