THE URINARY-EXCRETION OF THE COLLAGEN DEGRADATION MARKERS PYRIDINOLINE AND DEOXYPYRIDINOLINE IN AN EXPERIMENTAL RAT MODEL OF ALCOHOLIC BONE-DISEASE
THE URINARY-EXCRETION OF THE COLLAGEN DEGRADATION MARKERS PYRIDINOLINE AND DEOXYPYRIDINOLINE IN AN EXPERIMENTAL RAT MODEL OF ALCOHOLIC BONE-DISEASE
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DOI：
10.1093/oxfordjournals.alcalc.a045100
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发表时间：
1991-01-01
影响因子：
2.8
通讯作者：
BLACK, D
中科院分区：
文献类型：
--
作者：
PREEDY, VR;SHERWOOD, RA;BLACK, D
(1) 通过分析尿液中胶原蛋白、吡啶啉（PYD；主要存在于软骨和骨的胶原蛋白中，少量存在于其他组织中）和脱氧吡啶啉（DPD；仅存在于骨和牙本质的 I 型胶原蛋白中）的吡啶交联，研究了长期（6 周）乙醇喂养对大鼠全身骨骼组织的影响。 (2) 尿中总 PYD、游离 PYD 和结合 PYD 的浓度未因饲喂乙醇而改变。 相反，DPD 总浓度和结合 DPD 浓度显着降低。 游离DPD浓度的降低没有达到统计学显着性。 尿中总形式和结合形式的 PYD/DPD 摩尔比增加。 (3) 饮酒导致24小时尿PYD排泄总量略有下降，下降了15%。 对游离和结合形式 PYD 的排泄，以及游离/总、游离/结合和结合/总摩尔比没有统计学上的显着影响。 相比之下，总 DPD、游离 DPD 和结合 DPD 的 24 小时尿排泄量显着减少 25-55%。 此外，游离/总和游离/共轭摩尔比分别显着增加40%和80%，共轭/总摩尔比显着降低16%。 (4) 血浆电解质、酶和代谢物的分析数据并不支持以下观点：对胶原蛋白降解的影响是由于饮酒导致继发性器官功能障碍的结果。 (5) 结果表明，6 周的长期乙醇喂养对骨骼组织具有主要影响。 涉及骨吸收绝对速率的降低，并且乙醇可能抑制成熟交联的正常形成。
(1) The effect of chronic (6 weeks) ethanol feeding on whole-body skeletal tissue in the rat was studied by analysis of the urinary pyridinium crosslinks of collagen, pyridinoline (PYD; found predominantly in the collagens of cartilage and bone and to a lesser extent in other tissues) and deoxypyridinoline (DPD; found only in type I collagen of bone and dentine). (2) The urinary concentrations of total, free and conjugated PYD were unaltered by ethanol feeding. In contrast, there were significant reductions in total and conjugated DPD concentrations. The reduction in the concentration of free DPD did not achieve statistical significance. The urinary PYD/DPD molar ratios, of total and conjugated forms, were increased. (3) Alcohol feeding caused the total 24 hr urinary PYD excretion to fall slightly, by 15%. There were no statistically significant effects on excretion of free and conjugated forms of PYD, nor on the free/total, free/conjugated and conjugated/total molar ratios. In contrast, the 24 hr urinary excretion of total, free and conjugated DPD was significantly reduced by 25-55%. Furthermore, the free/total and free/conjugated molar ratios were significantly increased by 40% and 80%, respectively, and the conjugated/total molar ratio was significantly reduced by 16%. (4) Data from the analysis of plasma electrolytes, enzymes and metabolites did not support the contention that the effects on collagen degradation were a result of secondary organ dysfunction due to alcohol consumption. (5) The results suggest that chronic ethanol feeding for 6 weeks is having a primary effect on skeletal tissue. A reduction in the absolute rate of bone resorption is implicated and ethanol may inhibit the normal formation of the mature crosslinks.