Construction and characterisation of a BAC library made from field specimens of the onchocerciasis vector Simulium squamosum (Diptera: Simuliidae)
Construction and characterisation of a BAC library made from field specimens of the onchocerciasis vector Simulium squamosum (Diptera: Simuliidae)
复制标题
DOI：
10.1016/j.ygeno.2010.06.004
复制
发表时间：
2010-10-01
期刊：
影响因子：
4.4
通讯作者：
Post, R. J.
中科院分区：
文献类型：
--
作者：
Crainey, J. L.;Hurst, J.;Post, R. J.
以野生的人类盘尾丝虫病的重要传播媒介--该文库由12，288个BAC组成，平均插入片段大小为128 kb，预计含有约1.54 GB的克隆DNA。随机BAC-末端测序产生了超过95 kb的DNA序列数据，从这些数据中推测S.鳞状细胞癌基因序列和新的重复DNA家族，包括DNA转座子，反转录转座子和简单重复序列（SSR）。序列调查还提供了微生物来源的DNA的证据，并解剖样品黑蝇表明，一些用于制备文库的可能是由索科Isomermis lairdi寄生。与一组三个独立的黑蝇单拷贝基因和两个沃尔巴克氏体基因的杂交表明，该文库提供了约13倍的S。squamosum基因组和约12倍的覆盖率，其沃尔巴克氏体内共生体。皇冠版权所有（C）2010由爱思唯尔公司出版。All rights reserved.
A Bacterial Artificial Chromosome (BAC) library was made from wild-caught Simulium squamosum, which is an important vector of human onchocerciasis. The library is composed of 12,288 BACs, with an average insert size of 128 kb, and is expected to contain similar to 1.54 GB of cloned DNA. Random BAC-end sequencing generated over 95 kb of DNA sequence data from which putative S. squamosum gene sequences and novel repetitive DNA families were identified, including DNA transposons, retrotransposons and simple sequence repeats (SSRs). The sequence survey also provided evidence of DNA of microbial origin, and dissection of sample blackflies indicated that some of those used to prepare the library were likely to be parasitized by the mermithid Isomermis lairdi. Hybridisations with a set of three independent blackfly single-copy genes and two Wolbachia genes suggest that the library provides around 13-fold coverage of the S. squamosum genome and about 12-fold coverage of its Wolbachia endosymbiont. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.