SMK1, A DEVELOPMENTALLY-REGULATED MAP KINASE, IS REQUIRED FOR SPORE WALL ASSEMBLY IN SACCHAROMYCES-CEREVISIAE
SMK1, A DEVELOPMENTALLY-REGULATED MAP KINASE, IS REQUIRED FOR SPORE WALL ASSEMBLY IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1101/gad.8.18.2151
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发表时间：
1994-09-15
影响因子：
10.5
通讯作者：
WINTER, E
中科院分区：
文献类型：
--
作者：
KRISAK, L;STRICH, R;WINTER, E
丝裂原活化蛋白（MAP）激酶是一类保守的真核生物酶，介导对多种细胞外刺激的应答。在酵母中，不同的信号转导途径利用不同的MAP激酶家族成员。我们已经确定了一个新的酵母MAP激酶基因（命名为SMK 1），这是完成孢子形成所需的。分子和细胞学标记表明，减数分裂发育进行正常的纯合smk 1-Delta 1二倍体通过减数分裂II。然而，光学和电子显微镜显示smk 1子囊菌在组织孢子壁组装方面有缺陷。与孢子壁组装缺陷一致，SMK 1-Delta 1突变型ASCI显示对酶消化、热休克和暴露于乙醚的敏感性增强。SMK 1 mRNA，这是在营养细胞中检测不到的，是去阻遏至少200倍前原孢子外壳。我们认为SMK 1 MAP激酶参与了一个发育调节的信号转导途径，该途径协调细胞分化事件与转录程序。
Mitogen-activated protein (MAP) kinases comprise a family of conserved, eukaryotic enzymes that mediate responses to a wide variety of extracellular stimuli. In yeast, different signal transduction pathways utilize distinct MAP kinase family members. We have identified a new yeast MAP kinase gene (named SMK1) that is required for the completion of sporulation. Molecular and cytologic markers indicate that meiotic development proceeds normally in homozygous smk1-Delta 1 diploids through meiosis II. However, light and electron microscopy show that smk1 asci are defective in organizing spore wall assembly. Consistent with a defect in spore wall assembly, smk1-Delta 1 mutant asci display enhanced sensitivities to enzymatic digestion, heat shock, and exposure to ether. SMK1 mRNA, which is not detectable in vegetative cells, is derepressed at least 200-fold just prior to prospore enclosure. We propose that the SMK1 MAP kinase participates in a developmentally regulated signal transduction pathway that coordinates cytodifferentiation events with the transcriptional program.