Generalized cell morphological parameters based on interferometric phase microscopy and their application to cell life cycle characterization.
Generalized cell morphological parameters based on interferometric phase microscopy and their application to cell life cycle characterization.
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DOI：
10.1364/boe.3.001757
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发表时间：
2012-08-01
影响因子：
3.4
通讯作者：
Shaked NT
中科院分区：
文献类型：
--
作者：
Girshovitz P;Shaked NT
我们目前的分析工具，制定使用宽场干涉相位显微镜测量，并显示其能力，以独特的量化活癌细胞的生命周期。这些参数直接基于样品的光路延迟分布，并且不需要解耦单元干涉相位分布中的折射率和厚度，因此可以使用单帧采集来计算。为了证明这些参数的使用，我们构建了一个宽视场干涉相位显微镜设置，并密切跟踪HeLa癌细胞的整个生命周期。这些初步结果显示了这些参数区分细胞生命周期不同阶段以及其他生物现象的潜力。
We present analysis tools which are formulated using wide-field interferometric phase microscopy measurements, and show their ability to uniquely quantify the life cycle of live cancer cells. These parameters are based directly on the optical path delay profile of the sample and do not necessitate decoupling the refractive index and the thickness in the cell interferometric phase profile, and thus can be calculated using a single-frame acquisition. To demonstrate the use of these parameters, we have constructed a wide-field interferometric phase microscopy setup and closely traced the full lifecycle of HeLa cancer cells. These initial results show the potential of the parameters to distinguish between the different phases of the cell lifecycle, as well others biological phenomena.