Reassortment Between Divergent Strains of Camp Ripley Virus (Hantaviridae) in the Northern Short-Tailed Shrew (Blarina brevicauda).
Reassortment Between Divergent Strains of Camp Ripley Virus (Hantaviridae) in the Northern Short-Tailed Shrew (Blarina brevicauda).
复制标题
DOI：
10.3389/fcimb.2020.00460
复制
发表时间：
2020
影响因子：
5.7
通讯作者：
Yanagihara R
中科院分区：
文献类型：
--
作者：
Liphardt SW;Kang HJ;Arai S;Gu SH;Cook JA;Yanagihara R
节段RNA病毒株的基因组重配是一种重要的进化机制，可以产生对人类和动物健康具有深远影响的新型病毒，例如2009年由两种猪流感病毒重配引起的H1N1流感大流行。重配不限于流感病毒，并且已显示在布尼亚病毒目的成员中发生。大多数重配事件发生在密切相关的谱系之间，据称是由于病毒包装期间的分子限制。在最初的报道中，坎普里普利病毒（RPLV），一种新发现的汉坦病毒在北方短尾鼩（Blarina brevicauda），不同的基因组片段之间的系统发育不一致建议重配。我们已经扩大了采样，包括RPLV序列扩增档案组织的36个北方短尾鼩收集在12个州（阿肯色州，爱荷华州，堪萨斯州，马里兰州，马萨诸塞州，密歇根州，明尼苏达州，新罕布什尔州，俄亥俄州，宾夕法尼亚州，弗吉尼亚州，威斯康星州），和一个南方短尾鼩（Blarina carolinensis）从佛罗里达州，在美国。使用贝叶斯系统发育分析和基于图不相容性的重配分析，我们确定了多个实例的重配，跨越汉坦病毒科系统发育树，包括三个高度分歧，共循环谱系的M段，重配与一个保守的L段在多个人口的B。短尾的除了确定第一个已知的mobatvirus-like M-段序列从soricid主机，只有第二个从eulipotyphlan哺乳动物，我们的研究结果表明，重配可能是常见的不同的病毒株之间，并提供强有力的理由扩大空间，时间和分类学分析的分段病毒。
Genomic reassortment of segmented RNA virus strains is an important evolutionary mechanism that can generate novel viruses with profound effects on human and animal health, such as the H1N1 influenza pandemic in 2009 arising from reassortment of two swine influenza viruses. Reassortment is not restricted to influenza virus and has been shown to occur in members of the order Bunyavirales. The majority of reassortment events occurs between closely related lineages purportedly due to molecular constraints during viral packaging. In the original report of Camp Ripley virus (RPLV), a newfound hantavirus in the northern short-tailed shrew (Blarina brevicauda), phylogenetic incongruence between different genomic segments suggested reassortment. We have expanded sampling to include RPLV sequences amplified from archival tissues of 36 northern short-tailed shrews collected in 12 states (Arkansas, Iowa, Kansas, Maryland, Massachusetts, Michigan, Minnesota, New Hampshire, Ohio, Pennsylvania, Virginia, Wisconsin), and one southern short-tailed shrew (Blarina carolinensis) from Florida, within the United States. Using Bayesian phylogenetic analysis and Graph-incompatibility-based Reassortment Finder, we identified multiple instances of reassortment that spanned the Hantaviridae phylogenetic tree, including three highly divergent, co-circulating lineages of the M segment that have reassorted with a conserved L segment in multiple populations of B. brevicauda. In addition to identifying the first known mobatvirus-like M-segment sequences from a soricid host and only the second from a eulipotyphlan mammal, our results suggest that reassortment may be common between divergent virus strains and provide strong justification for expanded spatial, temporal, and taxonomic analyses of segmented viruses.