Intestinal epithelial cell proliferation and migration in Atlantic salmon, Salmo salar L.: effects of temperature and inflammation
Intestinal epithelial cell proliferation and migration in Atlantic salmon, Salmo salar L.: effects of temperature and inflammation
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DOI：
10.1007/s00441-013-1631-9
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发表时间：
2013-07-01
影响因子：
3.6
通讯作者：
Krogdahl, Ashild
中科院分区：
文献类型：
--
作者：
Chikwati, Elvis M.;Gu, Jinni;Krogdahl, Ashild
对大西洋鲑鱼(Salmo salar L.)的肠道上皮细胞(IEC)进行了为期28天的饲养试验。在海水中爆炸。四组鱼在8A或12A摄氏度的两种温度下饲养，并饲喂两种不同的饲料。这些饮食包括以玉米面筋和鱼粉为基础的参考饮食(FM)和含有20%提取豆粕(SBM)的实验性肠病饮食(SBM)。用体内增殖标记物5-溴-2‘-脱氧尿苷(BrdU)标记细胞，观察IEC的增殖和迁移情况。以增殖细胞核抗原(增殖细胞核抗原)标记为对照鉴定增殖细胞。在实验期间的5个时间点(3h~28d)采集近端(PI)、中端(MI)和远端(DI)的肠组织标本。组织学上，饲喂FM的鱼有正常的粘膜，而饲喂SBM的鱼出现DI肠病。所有肠区的粘膜皱折基底部均可见主要细胞增殖带。随着时间的推移，BrdU标记的细胞沿着粘膜皱折向上迁移到末端，然后消失。迁移率取决于肠道区域、温度和饮食。在摄食FM的鱼类中，PI的迁移率最高，MI和DI次之。饮食和温度对PI和MI的迁移几乎没有影响。在DI中的迁移对食物和温度最敏感，SBM和较高的水温都会增加增殖和迁移速度。DI中IEC的缓慢周转可能有助于解释该区域对饮食SBM诱导的肠病的敏感性。
A 28-day feeding trial was carried out to characterise intestinal epithelial cell (IEC) turnover in Atlantic salmon (Salmo salar L.) post-smolts in seawater. Four groups of fish raised at two temperatures of 8A degrees C or 12A degrees C and fed two different diets were investigated. The diets included a reference maize gluten and fishmeal-based diet (FM) and an experimental enteropathy-causing diet containing 20% extracted soybean meal (SBM). IEC proliferation and migration were investigated by labelling cells with the in vivo proliferation marker 5-bromo-2'-deoxyuridine (BrdU). Proliferating cell nuclear antigen (PCNA) labelling was used as a control for identifying proliferating cells. Samples of the proximal (PI), mid (MI) and distal (DI) intestinal regions were collected at five time points (3 h-28 days) over the experimental period. Histologically, FM-fed fish had normal mucosa, whereas the SBM-fed fish developed DI enteropathy. Major zones of cell proliferation were observed in the mucosal fold bases for all intestinal regions. Over time, BrdU-labelled cells migrated up mucosal folds to the tips before being lost. Migration rates were dependent on intestinal region, temperature and diet. Highest migration rates were observed in the PI followed by the MI and DI for FM-fed fish. Diet and temperature barely affected migration in the PI and MI. Migration in the DI was most sensitive to diet and temperature, with both SBM and the higher water temperature increasing proliferation and migration rates. The slow IEC turnover in the DI might help to explain the sensitivity of this region to dietary SBM-induced enteropathy.