Effects of Tsetse (Glossina morsitans morsitans Westw.) (Diptera: Glossinidae) Salivary Gland Homogenate on Coagulation and Fibrinolysis
Effects of Tsetse (Glossina morsitans morsitans Westw.) (Diptera: Glossinidae) Salivary Gland Homogenate on Coagulation and Fibrinolysis
复制标题
采采蝇（Glossina morsitans morsitans Westw.）（双翅目：舌蝇科）唾液腺匀浆对凝血和纤溶的影响
作者：
K. Parker;M. Mant
摘要采采蝇Glossina morsitans Westwood的唾液具有抗凝血酶抗凝活性并抑制凝血酶的酯水解活性。它没有其他可检测的抗凝剂特性。该抗凝剂在Sephadex级分上以单峰洗脱，立即起作用，热和储存稳定，分子量为11- 13，000。与肝素不同，它不被硫酸鱼精蛋白或甲苯胺蓝中和，也不需要辅因子抗凝血酶III来获得最佳抗凝活性。它具有与水蛭素相似的性质，但在Sephadex分级分离时不与蛋白质峰重叠，并且具有略微不同的分子量。唾液腺匀浆既不含纤溶酶原激活剂，也不含纤溶活性。用于维持舌蝇的兔血清含有抗唾液的沉淀抗体，在体外不能中和唾液抗凝剂。这种抗凝剂的性质表明，它可能是一个潜在的有用的抗血栓剂在人。
Summary The saliva of the tsetse, Glossina morsitans morsitans Westwood, has antithrombin anticoagulant activity and inhibits thrombin’s esterolytic activity. It has no other detectable anticoagulant properties. The anticoagulant elutes in a single peak on Sephadex fraction, is immediately acting, heat and storage stable, and has a molecular weight of 11-13,000. Unlike heparin it is not neutralized by protamine sulphate or toluidine blue and does not require the co-factor, antithrombin III, for optimal anticoagulant activity. It has similar properties to hirudin, but does not elute with a protein peak upon Sephadex fractionation and has a slightly different molecular weight. Salivary gland homogenates contained neither a plasminogen activator nor fibrinolytic activity. The sera of rabbits used to maintain tsetses, which contained precipitating antibodies against saliva, did not neutralize the salivary anticoagulant in vitro. The properties of this anticoagulant suggest that it might be a potentially useful antithrombotic agent in man.