Deep Grouping Analysis of the Altered Cervical Canal Microbiota in Intrauterine Adhesion Patients
Deep Grouping Analysis of the Altered Cervical Canal Microbiota in Intrauterine Adhesion Patients
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DOI：
10.1007/s43032-022-01006-w
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发表时间：
2022-06-16
影响因子：
2.9
通讯作者：
Li, Jingyun
中科院分区：
文献类型：
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作者：
Hua, Xiangdong;Xu, Lu;Li, Jingyun
为了深入分析宫内粘连（IUA）患者宫颈管菌群的变化及其与宫内粘连（IUA）严重程度的关系，我们前瞻性地连续招募了23例轻重度宫内粘连患者和8例不孕症患者、3例粘膜下肌瘤患者、8例子宫内膜息肉患者作为非IUA受试者。为进行深度分组分析，将入组妇女分别分为6组、2组和4组。从每个参与者的宫颈管中抽取宫颈粘液。通过16S rDNA高通量测序鉴定细菌组成。对6组患者进行分析，轻度IUA患者与粘膜下肌瘤患者具有相似的宫颈菌群多样性和组成。与轻度IUA参与者相比，中度或重度IUA患者的细菌多样性显著降低，厚壁菌门负荷较高。对于两组的分析，IUA患者的细菌多样性明显低于非IUA患者，而厚壁菌门的负荷明显高于非IUA患者。KEGG通路功能分析显示，在所有入组患者中，这些宫颈微生物群的代谢途径、次生代谢物的生物合成以及不同环境下的微生物代谢大多丰富。IUA的严重程度与宫颈管中厚壁菌门/不动杆菌门或乳酸菌属/加德纳菌属的丰度改变有关。宫颈管中较高的细菌负荷和较少的多样性可能与IUA的严重程度有关。这些宫颈菌群的功能主要参与代谢途径。
To deeply analyze the alterations of cervical canal microbiota in intrauterine adhesion (IUA) patients and microbiota's relation to intrauterine adhesion (IUA) severity, we prospectively enrolled 23 consecutive patients diagnosed with mild-to-severe IUA and 8 women with infertility, 3 women with submucous myomas, or 8 women with endometrial polyps, but without IUA, as non_IUA subjects. For deep grouping analysis, these enrolled women were divided into six groups, two groups, and four groups respectively. Cervical mucus was drawn from the cervical canal of each participant. The bacterial composition was identified by 16S rDNA high-throughput sequencing. For analysis of six groups, mild IUA patients had similar cervical canal microbiota diversity and composition with submucous myomas patients. Compared with mild IUA participants, patients with moderate or severe IUA had a significantly lower diversity of bacteria and higher load of Firmicutes. For analysis of two groups, IUA patients had a significantly lower diversity of bacteria and higher load of Firmicutes than non_IUA subjects. KEGG pathway function analysis showed that metabolic pathways, biosynthesis of secondary metabolites, and microbial metabolism in diverse environments were mostly enriched for these cervical canal microbiota in all enrolled patients. The severity of IUA was associated with the altered abundance of phylum Firmicutes/Acinetobacteria or genus Lactobacillus/Gardnerella in the cervical canal. Higher bacterial load but less diversity in the cervical canal may be related with the severity of IUA. The function of these cervical canal microbiota were mostly involved in metabolic pathways.