Genetic polymorphisms in pri-let-7a-2 are associated with ischemic stroke risk in a Chinese Han population from Liaoning, China: a case-control study.
Genetic polymorphisms in pri-let-7a-2 are associated with ischemic stroke risk in a Chinese Han population from Liaoning, China: a case-control study.
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pri-let-7a-2 基因多态性与中国辽宁汉族人群缺血性中风风险相关：一项病例对照研究
DOI：
10.4103/1673-5374.301019
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发表时间：
2021-07
影响因子：
6.1
通讯作者：
Wang YZ
中科院分区：
文献类型：
--
作者：
Wang YY;Zhang HY;Jiang WJ;Liu F;Li L;Deng SM;He ZY;Wang YZ
缺血性脑卒中是一种复杂的疾病，其发病机制与遗传多态性的发生有关。有证据表明，microRNA let-7a参与缺血性卒中的发病机制。Pri-miRNA是主要的转录物，它经历了几个加工步骤来产生pre-miRNA和后来的成熟mirna。在本病例对照研究中，我们分析了pri-let-7a-2变异在缺血性卒中高危患者中的分布，以及pri-let-7a-2变异与环境因素的相互作用。收集2013年12月至2015年12月中国医科大学第一附属医院1086例缺血性脑卒中患者和836例健康对照者的血液样本和临床资料。我们发现rs1143770 CC基因型和C等位基因与缺血性卒中风险降低相关，而rs629367 CC基因型与缺血性卒中风险增加相关。此外，这两个单核苷酸多态性在本研究样本中处于连锁不平衡状态。我们分析了基因与环境的相互作用，发现rs1143770与饮酒、吸烟和高血压史一起对缺血性卒中的发病机制起综合作用。因此，检测prilet -7a-2多态性可能会增加对缺血性卒中风险的认识。本研究于2012年2月20日获得中国医科大学第一附属医院机构伦理委员会批准（批准号：2012-38-1），并于2017年12月27日在中国临床试验注册中心注册（注册号：ChiCTR-COC-17013559）。
Ischemic stroke is a complicated disease, and its pathogenesis has been attributed to the occurrence of genetic polymorphisms. Evidence has suggested that the microRNA let-7a is involved in the pathogenesis of ischemic stroke. Pri-miRNA is the primary transcript, which undergoes several processing steps to generate pre-miRNA and, later, mature miRNAs. In this case-control study, we analyzed the distribution of pri-let-7a-2 variants in patients at a high risk for ischemic stroke and the interactions of pri-let-7a-2 variants and environmental factors. Blood samples and clinical information were collected from 1086 patients with ischemic stroke and 836 healthy controls between December 2013 and December 2015 at the First Affiliated Hospital of China Medical University. We found that the rs1143770 CC genotype and the C allele were associated with a decreased risk of ischemic stroke, whereas the rs629367 CC genotype was associated with an increased risk for ischemic stroke. Moreover, these two single-nucleotide polymorphisms were in linkage disequilibrium in this study sample. We analyzed gene-environment interactions and found that rs1143770 exerted a combined effect on the pathogenesis of ischemic stroke, together with alcohol use, smoking, and a history of hypertension. Therefore, the detection of pri-let-7a-2 polymorphisms may increase the awareness of ischemic stroke risk. This study was approved by the Institutional Ethics Committee of the First Affiliated Hospital of China Medical University, China (approval No. 2012-38-1) on February 20, 2012, and was registered with the Chinese Clinical Trial Registry (registration number: ChiCTR-COC-17013559) on December 27, 2017.