Activation of AKT is associated with metastasis of nasopharyngeal carcinoma
Activation of AKT is associated with metastasis of nasopharyngeal carcinoma
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DOI：
10.1007/s13277-011-0272-4
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发表时间：
2012-02-01
期刊：
影响因子：
--
通讯作者：
Guan, Jian
中科院分区：
文献类型：
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作者：
Liu, Ying;Chen, Long-Hua;Guan, Jian
尽管早期鼻咽癌的放疗效果优于其他肿瘤，但仍有一部分鼻咽癌患者在治疗后5年内死亡；其根本机制仍有待进一步了解。本研究旨在探讨鼻咽癌细胞逃避辐射的机制。本研究纳入了 I 期 NPC 患者。所有患者均接受放射治疗。在放疗开始前和放疗开始后 1 周对每位患者进行鼻咽癌活检。通过蛋白质印迹评估 AKT 在 NPC 组织中的表达。 NPC 细胞系 SUNE-1 细胞经过辐射处理。还评估了 SUNE-1 细胞中的 AKT 水平。通过流式细胞术评估SUNE-1细胞凋亡的频率。放射治疗后鼻咽癌组织中AKT水平显着升高，与鼻咽癌转移和死亡率显着相关。照射后，NPC 细胞系 SUNE-1 细胞表达的 AKT 水平高于对照细胞。 SUNE-1 细胞中 AKT 的敲低显着增加了细胞凋亡率。放射治疗可以增加 NPC 细胞中与 NPC 转移和死亡率增加相关的 AKT 水平。
Although radiotherapy results of nasopharyngeal carcinoma (NPC) at an early stage are better than other tumors, there is still a portion of patients with NPC who die before 5 years after the treatment; the underlying mechanism remains to be further understood. This study aims to investigate the mechanism by which NPC cells escape from irradiation. Patients with NPC at stage I was included in this study. All the patients were treated with irradiation. NPC biopsies were obtained from each patient before and 1 week after the start of radiotherapy. Expression of AKT in NPC tissue was assessed by Western blotting. NPC cell line, SUNE-1 cells, was treated with irradiation. The levels of AKT in SUNE-1 cells were also assessed. The frequency of apoptotic SUNE-1 cells was evaluated by flow cytometry. The levels of AKT were markedly increased in NPC tissue after treatment with irradiation, which was significantly correlated with NPC metastasis and mortality. After irradiation, NPC cell line, SUNE-1 cells, expressed higher levels of AKT than control cells. The knockdown of AKT in SUNE-1 cells markedly increased apoptotic cell rate. Radiotherapy can increase the levels of AKT in NPC cells that are associated with NPC metastasis and increase in mortality.