Linalool- and α-terpineol-induced programmed cell death in chlamydomonas reinhardtii
Linalool- and α-terpineol-induced programmed cell death in chlamydomonas reinhardtii
复制标题
芳樟醇和α-萜品醇诱导莱茵衣藻程序性细胞死亡
DOI：
10.1016/j.ecoenv.2018.10.062
复制
发表时间：
2019-01-15
影响因子：
6.8
通讯作者：
Zuo, Zhaojiang
中科院分区：
文献类型：
--
作者：
Chen, Yueting;Weng, Yuanyuan;Zuo, Zhaojiang
植物化感物质能有效抑制和/或控制藻类生长，有作为除藻剂使用的潜力。为了揭示从樟树提取物中鉴定出的两种抗藻化合物芳樟醇和α - 松油醇的致死机制，并促进它们作为除藻剂的开发，对经这两种化合物处理的莱茵衣藻的过氧化氢产生、光合能力、类半胱天冬酶活性、细胞核变化和DNA降解情况进行了研究。芳樟醇处理0.5小时和α - 松油醇处理1小时时，过氧化氢含量爆发式增加，与0小时相比分别增加了2.7倍和1.3倍。光合色素逐渐降解，Fv/Fm逐渐降至零，这表明细胞死亡不是由于生理过程逐渐消失而导致的坏死。在莱茵衣藻细胞中，用这两种化合物处理1小时后，类半胱天冬酶 - 9和类半胱天冬酶 - 3被激活。随着处理时间的延长，经DAPI染色的细胞核荧光强度逐渐增强然后减弱，从细胞中分离出的基因组DNA逐渐降解。这些特征表明，莱茵衣藻细胞在芳樟醇和α - 松油醇处理下的死亡是由活性氧（ROS）增加引发的程序性细胞死亡（PCD）。与α - 松油醇处理相比，在同一时间点，芳樟醇处理对PCD的促进作用更强，这可能是由于较高的ROS含量在短时间内诱导了较高的类半胱天冬酶 - 9和类半胱天冬酶 - 3活性。
Plant allelochemicals effectively inhibit and/ or control algal growth, and have potential to use as algaecide. To uncover the lethal mechanism of 2 anti-algal compounds linalool and alpha-terpineol identified from Cinnamomum camphora extracts, and promote their development as algaecide, the H2O2 production, photosynthetic abilities, caspase-like activities, nuclear changes and DNA degradation were investigated in Chlamydomonas reinhardtii treated with the 2 compounds. H2O2 content burst in linalool treatment at 0.5 h and in alpha-terpineol treatment at 1 h, with increases of 2.7 folds and 1.3 folds, respectively, compared to that at 0 h. The photosynthetic pigments gradually degraded, and Fv/Fm gradually declined to zero, indicating that the cell death was not a necrosis due to the gradual disappearance of physiological process. In C. reinhardtii cells, the caspase-9-like and caspase-3-like were activated in the treatments with the 2 compounds for 1 h. With prolonging the treatment time, the fluorescent intensity of the cell nucleuses stained by DAPI gradually enhanced and then faded, and the genomic DNA isolated from the cells gradually degraded. These hallmarks indicated that the death of C. reinhardtii cells in linalool and alpha-terpineol treatments was a programmed cell death (PCD) triggered by the increased reactive oxygen species (ROS). Compared to alpha-terpineol treatment, linalool treatment showed stronger promoting effects on PCD at the same time point, which may be caused by the higher ROS content inducing higher caspase-9-like and caspase-3-like activities in a short time.