TEMPORAL PHOSPHORYLATION OF THE DROSOPHILA PERIOD PROTEIN
TEMPORAL PHOSPHORYLATION OF THE DROSOPHILA PERIOD PROTEIN
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DOI：
10.1073/pnas.91.6.2260
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发表时间：
1994-03-15
影响因子：
11.1
通讯作者：
ROSBASH, M
中科院分区：
文献类型：
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作者：
EDERY, I;ZWIEBEL, LJ;ROSBASH, M
黑腹果蝇（Drosophila melanogaster）需要周期基因（per）来表现昼夜节律（类似或等于24小时）。我们在这里报告，每蛋白（PER）经历了每日振荡的表观分子量以及丰度。迁移率的变化主要或完全是由于多个磷酸化事件。经典的短周期形式的PER（PERS）的时间分布以与突变株的行为表型一致的方式改变。由于丰度和磷酸化的变化在恒定的环境条件下持续存在，它们反映或有助于自由运行的节奏。我们认为PER的磷酸化状态是果蝇生物钟计时机制中的一个重要决定因素。
The period gene (per) is required for Drosophila melanogaster to manifest circadian (similar or equal to 24 hr) rhythms. We report here that per protein (PER) undergoes daily oscillations in apparent molecular mass as well as abundance. The mobility changes are largely or exclusively due to multiple phosphorylation events. The temporal profile of the classic short-period form of PER (PERS) is altered in a manner consistent with the mutant strain's behavioral phenotype. As changes in abundance and phosphorylation persist under constant environmental conditions, they reflect or contribute to a free-running rhythm. We suggest that the phosphorylation status of PER is an important determinant in the Drosophila clock's time-keeping mechanism.