CELLULAR AND SUBCELLULAR HETEROGENEITY OF [CA-2+]I IN SINGLE HEART-CELLS REVEALED BY FURA-2
CELLULAR AND SUBCELLULAR HETEROGENEITY OF [CA-2+]I IN SINGLE HEART-CELLS REVEALED BY FURA-2
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DOI：
10.1126/science.3798114
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发表时间：
1987-01-16
期刊：
影响因子：
56.9
通讯作者：
LEDERER, WJ
中科院分区：
文献类型：
--
作者：
WIER, WG;CANNELL, MB;LEDERER, WJ
来自单个心肌细胞的钙指示剂信号（fura-2 荧光）的数字成像揭示了细胞质钙离子浓度（[Ca2+]i）的不同亚细胞模式，这些模式与不同类型的细胞外观和行为相关。在任何酶法分离的大鼠心脏细胞群中，都存在 (i) 机械静止细胞，其中 [Ca2+]i 空间均匀、随时间恒定且相对较低； (ii) 自发收缩细胞，其[Ca2+]i增加，但其中[Ca2+]i的空间均匀性被高[Ca2+]i的自发传播波周期性地中断； (iii) 过度收缩（四舍五入）且 [Ca2+]i 水平高于其他两种类型的细胞。分离细胞中观察到的 [Ca2+]i 的细胞和亚细胞异质性表明，应谨慎解释对细胞悬浮液进行的实验。先前在多细胞制剂中观察到的无空间分辨率的自发[Ca2+]i波动实际上在亚细胞水平上可能是不均匀的。
Digital imaging of calcium indicator signals (fura-2 fluorescence) from single cardiac cells has revealed different subcellular patterns of cytoplasmic calcium ion concentration ([Ca2+]i) that are associated with different types of cellular appearance and behavior. In any population of enzymatically isolated rat heart cells, there are (i) mechanically quiescent cells in which [Ca2+]i is spatially uniform, constant over time, and relatively low; (ii) spontaneously contracting cells, which have an increased [Ca2+]i, but in which the spatial uniformity of [Ca2+]i is interrupted periodically by spontaneous propagating waves of high [Ca2+]i; and (iii) cells that are hypercontracted (rounded up) and that have higher levels of [Ca2+]i than the other two types. The observed cellular and subcellular heterogeneity of [Ca2+]i in isolated cells indicates that experiments performed on suspensions of cells should be interpreted with caution. The spontaneous [Ca2+]i fluctuations previously observed without spatial resolution in multicellular preparations may actually be inhomogeneous at the subcellular level.