Global spread of carbapenem-resistant Acinetobacter baumannii
Global spread of carbapenem-resistant Acinetobacter baumannii
复制标题
DOI：
10.1093/jac/dkp428
复制
发表时间：
2010-02-01
影响因子：
5.2
通讯作者：
Seifert, Harald
中科院分区：
文献类型：
--
作者：
Higgins, Paul G.;Dammhayn, Cathrin;Seifert, Harald
我们调查了492株亚胺培南不敏感鲍曼不动杆菌全球分离株（北美和拉丁美洲、欧洲、亚洲、南非和澳大利亚）中碳青霉烯酶基因的分子流行病学和分布。通过PCR研究是否存在碳青霉烯酶编码基因。采用重复序列PCR（rep-PCR; DiversiLab）、序列型多重PCR和PFGE进行分子流行病学分析，亚胺培南不敏感性与内源性bla（OXA-51-like）或获得性碳青霉烯酶bla（OXA-23-like）、bla（OXA-40-like）或bla（OXA-58-like）上游的ISAba 1相关。分离物被分成8个不同的簇，包括欧洲克隆I、II和III。欧洲克隆II是最大的（246个分离株）和分布最广的群体（美国、泛欧、以色列、亚洲、澳大利亚和南非）。获得性OXA酶广泛分布，但不是唯一的碳青霉烯类耐药决定因素。鲍曼不动杆菌。
We have investigated the molecular epidemiology and distribution of carbapenemase genes in 492 imipenem-non-susceptible Acinetobacter baumannii worldwide isolates (North and Latin America, Europe, Asia, South Africa and Australia).MICs were determined by broth microdilution and Etest. The presence of carbapenemase-encoding genes was investigated by PCR. Molecular epidemiology was performed by repetitive sequence-based PCR (rep-PCR; DiversiLab), sequence-type multiplex PCR and PFGE.Imipenem non-susceptibility was associated with ISAba1 upstream of the intrinsic bla(OXA-51-like) or the acquired carbapenemase bla(OXA-23-like), bla(OXA-40-like) or bla(OXA-58-like). Isolates were grouped into eight distinct clusters including European clones I, II and III. European clone II was the largest (246 isolates) and most widespread group (USA, pan-Europe, Israel, Asia, Australia and South Africa).The global dissemination of eight carbapenem-resistant lineages illustrates the success this organism has had in epidemic spread. The acquired OXA enzymes are widely distributed but are not the sole carbapenem resistance determinant in A. baumannii.