The spatial expression patterns of DROOPING LEAF orthologs suggest a conserved function in grasses
The spatial expression patterns of DROOPING LEAF orthologs suggest a conserved function in grasses
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DOI：
10.1266/ggs.84.137
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发表时间：
2009-04-01
影响因子：
1.1
通讯作者：
Hirano, Hiro-Yuki
中科院分区：
文献类型：
--
作者：
Ishikawa, Motokazu;Ohmori, Yoshihiro;Hirano, Hiro-Yuki
垂叶基因调控水稻花心皮的形成和叶片中脉的形成。dl位点的功能缺失突变导致心皮向雄蕊同质转化，缺少中脉，导致垂叶表型。DL是YABBY基因家族的成员，与拟南芥（Arabidopsis thaliana）的螃蟹爪（CRC）基因密切相关。然而，拟南芥CRC的功能与水稻DL不同：它负责蜜腺发育，并部分参与心皮鉴定。因此，与DL/CRC相关的基因似乎在被子植物进化过程中功能多样化。为了评估DL功能在近缘物种中的保守性，我们研究了3种禾本科植物（玉米、小麦和高粱）中DL同源基因的原位表达模式，这些植物被划分为不同于包括苜蓿（O. sativa）在内的Ehrhartoideae亚科。结果表明，在花和叶发育过程中，三种植物DL同源基因的时空表达模式与水稻DL同源基因完全相同，表明DL相关基因在禾本科植物中具有功能保守性。在草科的祖先与草科的祖先分化后，DL可能被招募到草科的心皮形成和中脉形成中。
The DROOPING LEAF (DL) gene regulates carpel specification in the flower and midrib formation in the leaf in Oryza sativa (rice). Loss-of-function mutations in the dl locus cause homeotic transformation of carpels into stamens and lack of midrib, resulting in the drooping leaf phenotype. DL is a member of the YABBY gene family and is closely related to the CRABS CLAW (CRC) gene in Arabidopsis thaliana. The function of Arabidopsis CRC, however, differs from that of rice DL: it is responsible for nectary development and is partially involved in carpel identity. Thus, genes related to DL/CRC seem to have functionally diversified during angiosperm evolution. To assess the conservation of DL function in related species, here we examined the in situ expression patterns of DL orthologs in three grass species, i.e., maize, wheat and sorghum, which is assigned to subfamilies different from Ehrhartoideae including O. sativa. The results clearly show that the temporal and spatial expression patterns of DL orthologs in the three species are identical to those of rice DL in both flower and leaf development, suggesting that DL-related genes are functionally conserved within the grass family. It is likely that DL may have been recruited to carpel specification and midrib formation within the lineage of the grass family after divergence of their ancestor from that of eudicots.