Molecular cloning, mapping, and characterization of a novel human gene, MTA1-L1, showing homology to a metastasis-associated gene, MTA1
Molecular cloning, mapping, and characterization of a novel human gene, MTA1-L1, showing homology to a metastasis-associated gene, MTA1
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DOI：
10.1007/s100380050107
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发表时间：
1999-01-01
影响因子：
3.5
通讯作者：
Tokino, T
中科院分区：
文献类型：
--
作者：
Futamura, M;Nishimori, H;Tokino, T
通过对从人cDNA文库中随机选择的克隆进行大规模测序，我们分离出了一种新的人基因，其编码的产物与人MTA 1（一种与肿瘤侵袭和转移相关的蛋白质）的氨基酸序列具有59.6%的同一性。该cDNA全长2736个核苷酸，开放阅读框编码668个氨基酸，命名为MTA 1-L1（MTA 1 like 1）。在北方印迹上，MTA 1-L1的单个3.0-kb转录物普遍表达。MTA 1-L1基因的结构分析显示，18个外显子跨越8.1 kb的基因组DNA。我们通过荧光原位杂交将MTA 1-L1位点定位于染色体11 q12 -13.1带。
Through large-scale sequencing of clones randomly selected from libraries of human cDNAs, we have isolated a novel human gene encoding a product with 59.6% identity in amino acid sequence to human MTA1, a protein associated with tumor invasion and metastasis. This cDNA, named MTA1-L1 (MTA1 like 1), consists of 2736 nucleotides with an open reading frame encoding 668 amino acids. A single 3.0-kb transcript of MTA1-L1 was expressed ubiquitously on Northern blots. Structural analysis of the MTA1-L1 gene revealed 18 exons spanning 8.1 kb of genomic DNA. We assigned the MTA1-L1 locus to chromosomal band 11q12-13.1 by fluorescence in situ hybridization.