The function of the Drosophila fat facets deubiquitinating enzyme in limiting photoreceptor cell number is intimately associated with endocytosis.
The function of the Drosophila fat facets deubiquitinating enzyme in limiting photoreceptor cell number is intimately associated with endocytosis.
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发表时间：
2000-04
期刊：
影响因子：
4.6
通讯作者：
Angelica L. M. Cadavid;A. Ginzel;J. Fischer
中科院分区：
文献类型：
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作者：
Angelica L. M. Cadavid;A. Ginzel;J. Fischer
脂肪面是细胞通讯途径中必需的去泛素化酶，该途径将果蝇复眼每个面的光感受器细胞数量限制在8个。遗传数据支持一个模型，其中Faf去除泛素，蛋白质降解的多肽标签，从一个特定的泛素化蛋白质，从而防止其降解。在这里，液体面基因的突变被确定为脂肪面突变眼表型的显性增强子。液体面位点编码epsin，一种与网格蛋白内吞复合物相关的脊椎动物蛋白。遗传实验结果表明，脂肪面和液体面促进普通细胞的内吞作用和功能，产生抑制信号，阻止异位光感受器的决定。此外，研究表明，脂肪面突变表型对液体面表达水平异常敏感。我们认为液体小晶状体是眼内脂肪小晶状体的关键基质。
Fat facets is a deubiquitinating enzyme required in a cell communication pathway that limits to eight the number of photoreceptor cells in each facet of the Drososphila compound eye. Genetic data support a model whereby Faf removes ubiquitin, a polypeptide tag for protein degradation, from a specific ubiquitinated protein thus preventing its degradation. Here, mutations in the liquid facets gene were identified as dominant enhancers of the fat facets mutant eye phenotype. The liquid facets locus encodes epsin, a vertebrate protein associated with the clathrin endocytosis complex. The results of genetic experiments reveal that fat facets and liquid facets facilitate endocytosis and function in common cells to generate an inhibitory signal that prevents ectopic photoreceptor determination. Moreover, it is demonstrated that the fat facets mutant phenotype is extraordinarily sensitive to the level of liquid facets expression. We propose that Liquid facets is a candidate for the critical substrate of Fat facets in the eye.