Using Fluorescence Microscopy to Study Mitosis.
Using Fluorescence Microscopy to Study Mitosis.
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使用荧光显微镜研究有丝分裂。
DOI:
10.1007/978-1-4939-3542-0_1
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发表时间:
2016
期刊:
影响因子:
--
通讯作者:
Wadsworth,Patricia
中科院分区:
文献类型:
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作者:
Balchand,SaiK;Mann,BarbaraJ;Wadsworth,Patricia
Fluorescence microscopy is one of the most important approaches in the cell biologist’s toolbox for studying the mitotic spindle. In fact, many of the key insights into our understanding of mitosis have been enabled by the visualization of mitotic processes using fluorescence microscopy. Here, we summarize some of the important considerations for imaging mitosis using fluorescence microscopy. Because light can damage live cells, we emphasize the importance of minimizing cellular damage while obtaining informative images.