Using Fluorescence Microscopy to Study Mitosis.

Using Fluorescence Microscopy to Study Mitosis.
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使用荧光显微镜研究有丝分裂。

DOI:
10.1007/978-1-4939-3542-0_1
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发表时间:
2016
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
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通讯作者:
Wadsworth,Patricia
Wadsworth,Patricia
中科院分区:
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文献类型:
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作者:
Balchand,SaiK;Mann,BarbaraJ;Wadsworth,Patricia

文献摘要

相似文献

荧光显微镜是细胞生物学家研究有丝分裂纺锤体的最重要的方法之一。事实上,我们理解有丝分裂的许多关键见解都是通过使用荧光显微镜观察有丝分裂过程实现的。在这里,我们总结了一些使用荧光显微镜成像有丝分裂的重要考虑。由于光可以损伤活细胞,我们强调在获得信息图像的同时尽量减少细胞损伤的重要性。
Fluorescence microscopy is one of the most important approaches in the cell biologist’s toolbox for studying the mitotic spindle. In fact, many of the key insights into our understanding of mitosis have been enabled by the visualization of mitotic processes using fluorescence microscopy. Here, we summarize some of the important considerations for imaging mitosis using fluorescence microscopy. Because light can damage live cells, we emphasize the importance of minimizing cellular damage while obtaining informative images.