Co-operative Bmp- and Fgf-signaling inputs convert skin wound healing to limb formation in urodele amphibians
Co-operative Bmp- and Fgf-signaling inputs convert skin wound healing to limb formation in urodele amphibians
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DOI：
10.1016/j.ydbio.2014.09.021
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发表时间：
2014-12-01
影响因子：
2.7
通讯作者：
Satoh, Akira
中科院分区：
文献类型：
--
作者：
Makanae, Aki;Mitogawa, Kazumasa;Satoh, Akira
有尾目两栖动物具有显著的器官再生能力，其肢体再生能力作为一种代表性现象而被研究。在世纪早期，据报道神经是成功诱导肢体再生的重要组织。长期以来，人们一直在寻找在诱导肢体再生反应中起作用的神经物质。为了识别神经因素，建立了一种名为副肢模型CALM的新实验系统。有尾目两栖动物的皮肤创伤导致皮肤创伤愈合，但从未在肢体诱导。然而，在ALM中，神经偏向受伤的皮肤诱导肢体形成。因此，可以认为神经具有将皮肤伤口愈合转化为肢体形成的能力。在本研究中，合作BMP和FGF的应用，而不是神经偏差，受伤的皮肤转化皮肤伤口愈合肢体的形成，在两个urodele两栖动物，蝾螈（Ambystoma mexicanum）和蝾螈（Pleurodeles waltl）：我们的研究结果表明，定义的因素可以诱导同源异型转化urodele两栖动物的胚后体。BMP和Fgf（s）的组合可能有助于开发用于器官再生的新治疗方法。(C)2014作者爱思唯尔公司出版
Urodele amphibians have remarkable organ regeneration capability, and their limb regeneration capability has been investigated as a representative phenomenon. In the early 19th century, nerves were reported to be an essential tissue for the successful induction of limb regeneration. Nerve substances that function in the induction of limb regeneration responses have long been sought. A new experimental system called the accessory limb model CALM) has been established to identify the nerve factors. Skin wounding in urodele amphibians results in skin wound healing but never in limb induction. However, nerve deviation to the wounded skin induces limb formation in ALM. Thus, nerves can be considered to have the ability to transform skin wound healing to limb formation. In the present study, co-operative Bmp and Fgf application, instead of nerve deviation, to wounded skin transformed skin wound healing to limb formation in two urodele amphibians, axolotl (Ambystoma mexicanum) and newt (Pleurodeles waltl): Our findings demonstrate that defined factors can induce homeotic transformation in postembryonic bodies of urodele amphibians. The combination of Bmp and Fgf(s) may contribute to the development of novel treatments for organ regeneration. (C) 2014 The Authors. Published by Elsevier Inc.