Diastereoisomer- and Enantiomer-Specific Accumulation, Depuration, and Bioisomerization of Hexabromocyclododecanes in Zebrafish (Danio rerio)
Diastereoisomer- and Enantiomer-Specific Accumulation, Depuration, and Bioisomerization of Hexabromocyclododecanes in Zebrafish (Danio rerio)
复制标题
斑马鱼 (Danio rerio) 中六溴环十二烷的非对映异构体和对映异构体特异性积累、纯化和生物异构化
DOI：
10.1021/es302166p
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发表时间：
2012-10-16
影响因子：
11.4
通讯作者：
Zhang, Xian
中科院分区：
文献类型：
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作者：
Du, Miaomiao;Lin, Lifeng;Zhang, Xian
在这项研究中，将斑马鱼（Danio rerio）暴露于两种不同浓度的HBCD非对映异构体（α -、β -和γ -HBCD）中42天，然后食用清洁食物21天，以检测HBCD非对映异构体的生物积累、净化和对映异构体部分（EFs），并测试鱼类中HBCD的生物异构化。斑马鱼体内α -、β -和γ - hbcd的去除遵循一阶过程。三种非对映异构体的生物积累参数在低剂量和高剂量之间存在差异，表明它们的生物积累具有浓度依赖性。α - hbcd的计算同化效率（ae）、生物放大因子（BMFs）和半衰期（t(1/2)）在三种非对映异构体中最高。此外，研究表明斑马鱼可以将γ - hbcd生物转化为α - hbcd。α - hbcd的AE、BMF和t（1/2）最高以及γ - hbcd与α - hbcd的生物异构化可以解释α - hbcd在生物群样品中占优势的原因。在不同的生物积累和净化时间，斑马鱼体内α -和γ - hbcd的EFs均显著大于相应食物中的EFs (P < 0.05)，表明(+)α -对映体和(+)γ -对映体相对于(-)α -对映体和(-)γ -对映体分别选择性富集。
In this study, zebrafish (Danio rerio) were exposed to two dietary concentrations of individual HBCD diastereoisomers (alpha-, beta- and gamma-HBCD) for 42 days, followed by clean food for 21 days, to examine bioaccumulation, depuration, and enantiomer fractions (EFs) of HBCD diastereoisomers and to test the bioisomerization of 0, HBCDs in fish. The depuration of alpha-, beta-, and gamma-HBCD in zebrafish followed the first-order process. Bioaccumulation parameters of the three diastereoisomers differed between low and high dose, suggesting that the bioaccumulation of them is concentration dependent. Calculated assimilation efficiencies (AEs), biomagnification factors (BMFs), and half-lives (t(1/2)) of alpha-HBCD were the highest among the three diastereoisomers. Furthermore, the study showed that zebrafish could biotransform gamma-HBCD to alpha-HBCD. The highest AE, BMF, and t(1/2) of alpha-HBCD and bioisomerization of gamma-HBCD to alpha-HBCD could explain why alpha-HBCD appears to be dominant in biota samples. The EFs for alpha- and gamma-HBCD in zebrafish estimated at different times of bioaccumulation and depuration were all significantly greater than those in corresponding food (P < 0.05), indicating selective enrichment of (+) alpha-enantiomer and (+) gamma-enantiomer relative to (-) alpha-enantiomer and (-) gamma-enantiomer, respectively.