Variability and heritability of cell division pathways in Toxoplasma gondii
Variability and heritability of cell division pathways in Toxoplasma gondii
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DOI：
10.1242/jcs.01494
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发表时间：
2004-11-01
影响因子：
4
通讯作者：
Murray, JM
中科院分区：
文献类型：
--
作者：
Hu, K;Roos, DS;Murray, JM
弓形虫中稳定表达的组蛋白2b-YFP融合蛋白具有几个优点：它揭示了以前隐藏的核形态学细节;它使观察细胞周期事件成为可能;它为定量测量活细胞中的DNA含量提供了基础;并且它能够根据细胞周期阶段或倍性分选活细胞。利用这种细胞系，可以识别并直接克隆产生两个、三个或四个子细胞的不同细胞分裂模式产生的个体后代。这些实验证实，通过所有细胞分裂途径产生的后代都是有活力和有感染性的。此外，成熟寄生虫在细胞分裂期间产生的后代数量与其兄弟姐妹的数量密切相关。因此，细胞分裂途径的完整库由通过任何一个单独途径产生的单个细胞继承。这些结果扩大了正常T细胞分裂的范围，并为进一步研究这种重要的人类病原体的核结构和增殖提供了有用的工具。
A histone 2b-YFP fusion protein stably expressed in Toxoplasma gondii has several advantages: it reveals previously hidden details of nuclear morphology; it makes it possible to observe cell-cycle events; it provides a basis for quantitative measurements of DNA content in living cells; and it enables sorting of live cells according to cell-cycle phase or ploidy. With this cell line it was possible to recognize and directly clone individual progeny arising from different patterns of cell division that produce two, three or four daughter cells. These experiments established that the progeny produced by all cell division pathways are viable and infective. Furthermore, the number of progeny produced by a mature parasite during cell division is hot correlated with the number of its siblings. The complete repertoire of cell division pathways is therefore inherited by a single cell produced through any one of the individual paths. The results expand the range of what must be considered normal in T gondii cell division and provide a useful tool for further study of nuclear structure and proliferation in this important human pathogen.