Separate effects of ischemia and reperfusion on vascular permeability in ventilated ferret lungs.
Separate effects of ischemia and reperfusion on vascular permeability in ventilated ferret lungs.
复制标题
缺血和再灌注对通气雪貂肺血管通透性的单独影响。
DOI：
10.1152/jappl.1992.73.6.2616
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Sylvester,JT
中科院分区：
文献类型：
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作者：
Becker,PM;Pearse,DB;Permutt,S;Sylvester,JT
在全身器官中，当氧气重新输入到缺氧缺血组织中时，缺血-再灌注损伤被认为发生在再灌流期间。相反，在缺血期间，在再灌流之前，通风的肺可能更容易受到损伤，因为氧分压在缺血期间会很高，再灌流后会降低。为了评估这种可能性，我们比较了单纯高氧缺血和高氧缺血加常氧再灌流对雪貂肺血管通透性的影响。通透性是通过测量白蛋白的过滤系数(KF)和渗透反射系数(Sigma Alb)来估计的，使用不需要再灌流的方法进行这些测量。对照组(n=5)的KF和Sigma-Alb分别为0.033±0.004 g·min-1、1.100 g·min-1和0.69±0.07 g·min-1。这些值与在其他物种的正常活体肺中报道的值没有什么不同。在95%O2-5%CO2通气组中，观察短时间(54+/-9min，n=10)和长时间(180min，n=7)肺缺血的影响。KF和Sigma Alb在短时间缺血后无明显变化(Kf=0.051+/-0.006 g·min-1，Sigma Alb=1.100 g-1，Sigma Alb=0.69+/-0.07)，但在长时间缺血后显著增加(Kf=0.233+/-0.049 g.min-1×MmHg-1 x 100g-1，Sigma Alb=0.36+/-0.05)。
In systemic organs, ischemia-reperfusion injury is thought to occur during reperfusion, when oxygen is reintroduced to hypoxic ischemic tissue. In contrast, the ventilated lung may be more susceptible to injury during ischemia, before reperfusion, because oxygen tension will be high during ischemia and decrease with reperfusion. To evaluate this possibility, we compared the effects of hyperoxic ischemia alone and hyperoxic ischemia with normoxic reperfusion on vascular permeability in isolated ferret lungs. Permeability was estimated by measurement of filtration coefficient (Kf) and osmotic reflection coefficient for albumin (sigma alb), using methods that did not require reperfusion to make these measurements. Kf and sigma alb in control lungs (n = 5), which were ventilated with 14% O2–5% CO2 after minimal (15 +/- 1 min) ischemia, averaged 0.033 +/- 0.004 g.min-1.mmHg-1.100 g-1 and 0.69 +/- 0.07, respectively. These values did not differ from those reported in normal in vivo lungs of other species. The effects of short (54 +/- 9 min, n = 10) and long (180 min, n = 7) ischemia were evaluated in lungs ventilated with 95% O2–5% CO2. Kf and sigma alb did not change after short ischemia (Kf = 0.051 +/- 0.006 g.min-1.mmHg-1.100 g-1, sigma alb = 0.69 +/- 0.07) but increased significantly after long ischemia (Kf = 0.233 +/- 0.049 g.min-1 x mmHg-1 x 100 g-1, sigma alb = 0.36 +/- 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)