Comparison of Escherichia coli O157:H7 antigen detection in stool and broth cultures to that in sorbitol-MacConkey agar stool cultures.
Comparison of Escherichia coli O157:H7 antigen detection in stool and broth cultures to that in sorbitol-MacConkey agar stool cultures.
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粪便和肉汤培养物中大肠杆菌 O157:H7 抗原检测与山梨糖醇-麦康凯琼脂粪便培养物中大肠杆菌 O157:H7 抗原检测的比较。
DOI：
10.1128/jcm.38.9.3404-3406.2000
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发表时间：
2000
影响因子：
9.4
通讯作者：
Tarr,PI
中科院分区：
文献类型：
--
作者：
Stapp,JR;Jelacic,S;Yea,YL;Klein,EJ;Fischer,M;Clausen,CR;Qin,X;Swerdlow,DL;Tarr,PI
We evaluated the Meridian IC-STAT direct fecal and broth culture antigen detection methods with samples from children infected withEscherichia coliO157:H7 and correlated the antigen detection results with the culture results. Stools of 16 children who had recently had stool cultures positive for this pathogen (population A) and 102 children with diarrhea of unknown cause (population B) were tested with the IC-STAT device (direct testing). Fecal broth cultures were also tested with this device (broth testing). The results were correlated to a standard of the combined yield from direct culture of stools on sorbitol-MacConkey (SMAC) agar and culture of broth on SMAC agar. Eleven (69%) of the population A stool specimens yieldedE. coliO157:H7 when plated directly on SMAC agar. Two more specimens yielded this pathogen when the broth culture was similarly plated. Of these 13 stool specimens, 8 and 13 were positive by direct and broth testing (respective sensitivities, 62 and 100%). Compared to the sensitivity of a simultaneously performed SMAC agar culture, the sensitivity of direct testing was 73%. Three (3%) of the population B stool specimens containedE. coliO157:H7 on SMAC agar culture; one and three of these stool specimens were positive by direct and broth testing, respectively. The direct and broth IC-STAT tests were 100% specific with samples from children from population B. Direct IC-STAT testing of stools is rapid, easily performed, and specific but is insufficiently sensitive to exclude the possibility of infection withE. coliO157:H7. Performing the IC-STAT test with a broth culture increases its sensitivity. However, attempts to recoverE. coliO157:H7 by culture should not be abandoned but, rather, should be increased when the IC-STAT test result is positive.
