CEACAM1 isoforms with different cytoplasmic domains show different localization, organization and adhesive properties in polarized epithelial cells.
CEACAM1 isoforms with different cytoplasmic domains show different localization, organization and adhesive properties in polarized epithelial cells.
复制标题
具有不同胞质结构域的 CEACAM1 亚型在极化上皮细胞中表现出不同的定位、组织和粘附特性。
作者：
U. Sundberg;B. Obrink
CEACAM 1是一种在上皮细胞、血管内皮细胞和白细胞中表达的信号细胞粘附分子。其表达为具有不同胞质结构域的两种主要同种型。CEACAM 1在极化上皮细胞的细胞-细胞接触区域和顶端表面上都存在，但不知道不同的同种型如何分布在极化细胞中或CEACAM 1在顶端表面中的功能是什么。我们研究了本地化和组织的两个CEACAM 1亚型转染，极化MDCK细胞共聚焦显微镜和差异表面标记。CEACAM 1-L在顶面和侧面上都被发现，而CEACAM 1-S仅出现在顶面上。维持CEACAM 1-L的侧向定位需要相邻细胞上CEACAM 1-L分子之间的嗜同性结合。针对不同表位的抗CEACAM 1抗体的双标记表明顶端CEACAM 1-L以嗜同性粘附状态或游离非粘附状态发生。CEACAM 1-S几乎完全以嗜同性粘附状态出现。这些发现表明CEACAM 1介导根尖表面上相邻微绒毛之间的粘附键。
CEACAM1 is a signaling cell adhesion molecule expressed in epithelia, vessel endothelia and leukocytes. It is expressed as two major isoforms with different cytoplasmic domains. CEACAM1 occurs both in cell-cell contact areas and on apical surfaces of polarized epithelial cells, but it is not known how the different isoforms are distributed in polarized cells or what the functions of CEACAM1 are in the apical surfaces. We investigated the localization and organization of the two CEACAM1 isoforms in transfected, polarized MDCK cells by confocal microscopy and differential surface labelling. CEACAM1-L was found on both the apical and the lateral surfaces, whereas CEACAM1-S appeared exclusively on the apical surfaces. Maintenance of the lateral localization of CEACAM1-L required homophilic binding between CEACAM1-L molecules on adjacent cells. Double-labelling with anti-CEACAM1 antibodies directed against different epitopes indicated that apical CEACAM1-L occurred either in a homophilic adhesive state or in a free non-adhesive state. CEACAM1-S appeared almost exclusively in the homophilic adhesive state. These findings suggest that CEACAM1 mediates adhesive bonds between adjacent microvilli on the apical surfaces.
影响因子：
11.2
作者：
J. Hsieh;W. Luo;Weitao Song;Yang Wang;D. Kleinerman;Nguyen T. Van;S. Lin
通讯作者：
J. Hsieh;W. Luo;Weitao Song;Yang Wang;D. Kleinerman;Nguyen T. Van;S. Lin
CD66 家族成员与人类中性粒细胞中的酪氨酸激酶活性相关。
DOI：
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发表时间：
1995
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Skubitz,KM;Campbell,KD;Ahmed,K;Skubitz,AP
通讯作者：
Skubitz,AP